Kim Doyeon, Lee Chanho, Kim Manho, Park Ju Hyun
Department of Biomedical Science, Kangwon National University, Chuncheon-si 24341, Gangwon-do, Republic of Korea.
Institute of Molecular Science and Fusion Technology, Kangwon National University, Chuncheon-si 24341, Gangwon-do, Republic of Korea.
Antioxidants (Basel). 2024 Nov 29;13(12):1474. doi: 10.3390/antiox13121474.
Bone marrow mesenchymal stem cells (BM-MSCs) play a crucial role in bone formation through their ability to differentiate into osteoblasts. Aging, however, detrimentally affects the differentiation and proliferation capacities of BM-MSCs, consequently impairing bone regeneration. Thus, mitigating the aging effects on BM-MSCs is vital for addressing bone-related pathologies. In this study, we demonstrate that extracellular nanovesicles isolated from gold kiwi (GK-NVs) protect human BM-MSCs from ultraviolet (UV)-induced photoaging, thereby alleviating aging-related impairments in cellular functions that are crucial for bone homeostasis. Notably, GK-NVs were efficiently taken up by BM-MSCs without causing cytotoxicity. GK-NVs reduced intracellular reactive oxygen species (ROS) levels upon UV irradiation, restoring impaired proliferation and migration capabilities. Furthermore, GK-NVs corrected the skewed differentiation capacities of UV-irradiated BM-MSCs by enhancing osteoblast differentiation, as evidenced by the increased expression in osteoblast-specific genes and the calcium deposition, and by reducing adipocyte differentiation, as indicated by the decreased lipid droplet formation. These findings position GK-NVs as a promising biomaterial for the treatment of bone-related diseases such as osteoporosis.
骨髓间充质干细胞（BM-MSCs）通过其分化为成骨细胞的能力在骨形成中发挥关键作用。然而，衰老会对BM-MSCs的分化和增殖能力产生不利影响，从而损害骨再生。因此，减轻衰老对BM-MSCs的影响对于解决与骨相关的病理问题至关重要。在本研究中，我们证明从金奇异果中分离出的细胞外纳米囊泡（GK-NVs）可保护人BM-MSCs免受紫外线（UV）诱导的光老化，从而减轻对骨稳态至关重要的细胞功能中与衰老相关的损伤。值得注意的是，BM-MSCs能有效摄取GK-NVs且不会引起细胞毒性。UV照射后，GK-NVs降低了细胞内活性氧（ROS）水平，恢复了受损的增殖和迁移能力。此外，GK-NVs通过增强成骨细胞分化纠正了UV照射的BM-MSCs的分化能力偏差，这表现为成骨细胞特异性基因表达增加和钙沉积，同时通过减少脂滴形成表明其减少了脂肪细胞分化。这些发现表明GK-NVs是一种有前景的生物材料，可用于治疗骨质疏松症等与骨相关的疾病。
