Kim Jaewook, Kim Eiseul, Yang Seung-Min, Park Si Hong, Kim Hae-Yeong
Institute of Life Sciences & Resources, Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Department of Food Science and Technology, Oregon State University, Corvallis, OR 97331, USA.
Biomolecules. 2024 Dec 18;14(12):1624. doi: 10.3390/biom14121624.
This study introduces an innovative on-site diagnostic method for rapidly detecting the / complex (SBSEC), crucial for livestock health and food safety. Through a comprehensive genomic analysis of 206 genomes, this study identified genetic markers that improved classification and addressed misclassifications, particularly in genomes labeled and . These markers were integrated into a portable quantitative polymerase chain reaction (qPCR) that can detect SBSEC species with high sensitivity (down to 10 or 10 colony-forming units/mL). The portable system featuring a flat chip and compact equipment allows immediate diagnosis within 30 min. The diagnostic method was validated in field conditions directly from cattle udders, farm environments, and dairy products. Among the 100 samples, 51 tested positive for bacteria associated with mastitis. The performance of this portable qPCR was comparable to laboratory methods, offering a reliable alternative to whole-genome sequencing for early detection in clinical, agricultural, and environmental settings.
本研究介绍了一种创新的现场诊断方法，用于快速检测对牲畜健康和食品安全至关重要的/复杂菌（SBSEC）。通过对206个基因组进行全面的基因组分析，本研究确定了可改善分类并解决错误分类问题的遗传标记，特别是在标记为和的基因组中。这些标记被整合到一种便携式定量聚合酶链反应（qPCR）中，该反应能够以高灵敏度（低至10或10菌落形成单位/毫升）检测SBSEC菌种。这种具有扁平芯片和紧凑设备的便携式系统可在30分钟内实现即时诊断。该诊断方法在直接来自奶牛乳房、农场环境和乳制品的现场条件下得到了验证。在100个样本中，51个检测出与乳腺炎相关的细菌呈阳性。这种便携式qPCR的性能与实验室方法相当，为临床、农业和环境环境中的早期检测提供了一种可靠的全基因组测序替代方法。
