Reges Bianca Mara, da Silva Oliveira Francisca Andréa, Fonteles Thatyane Vidal, Rodrigues Sueli
Food Engineering Department, Federal University of Ceara, Fortaleza 60440-900, CE, Brazil.
Drug Research and Development Center, Federal University of Ceara, Fortaleza 60356-001, CE, Brazil.
Foods. 2024 Dec 20;13(24):4121. doi: 10.3390/foods13244121.
The present study evaluates the effects of açai juice containing gluco-oligosaccharides and dextran, fermented by NRRL B-41408 (synbiotic juice), on the human fecal microbiota. The juice is subjected to simulated digestion and fecal fermentation after production and 42 days of refrigerated storage. High throughput 16S rRNA sequencing and HPLC are used to identify the bacterial cells and metabolites. The results show that the viability of is stable during the refrigerated storage, indicating that the metabolism is maintained even under low temperatures and pH. Furthermore, gluco-oligosaccharides and dextran prove to be resistant to gastrointestinal conditions and are quickly consumed during fecal fermentation. The synbiotic açai juice enhances the microbial diversity and stimulates the production of short-chain fatty acids (SCFA), including acetate, propionate, and isobutyrate. Elevated propionate levels are directly associated with an increased abundance of , , , , and after 48 h of fermentation. This highlights the potential of synbiotic açai juice as a functional beverage, supported by the significant increase in microbial diversity reflected in the Shannon and Simpson's diversity indexes (Shannon = 116.6%, 117.2%, 125.15%, and 116.02%; Simpson's = 151.86%, 177.22%, 152.5%, and 163.73%).
本研究评估了由NRRL B - 41408发酵的含有低聚葡萄糖和葡聚糖的阿萨伊果汁（合生元果汁）对人体粪便微生物群的影响。该果汁在生产后以及冷藏储存42天后进行模拟消化和粪便发酵。使用高通量16S rRNA测序和高效液相色谱法来鉴定细菌细胞和代谢产物。结果表明，在冷藏储存期间其活力稳定，这表明即使在低温和低pH值条件下代谢仍能维持。此外，低聚葡萄糖和葡聚糖被证明对胃肠道环境具有抗性，并且在粪便发酵过程中迅速被消耗。合生元阿萨伊果汁增强了微生物多样性，并刺激了短链脂肪酸（SCFA）的产生，包括乙酸盐、丙酸盐和异丁酸盐。发酵48小时后，丙酸盐水平升高与[具体细菌种类]的丰度增加直接相关。这突出了合生元阿萨伊果汁作为功能性饮料的潜力，香农和辛普森多样性指数的显著增加也证明了这一点（香农指数分别为116.6%、117.2%、125.15%和116.02%；辛普森指数分别为151.86%、177.22%、152.5%和163.73%）。
