Li Xiaolan, Huang Jin, Li Na, Salah Mahmoud, Guan Shuoning, Pan Wenwen, Wang Ziyi, Zhou Xinghua, Wang Yun
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Department of Environmental Agricultural Science, Faculty of Graduate Studies and Environmental Research, Ain Shams University, Cairo 11566, Egypt.
Foods. 2024 Dec 20;13(24):4137. doi: 10.3390/foods13244137.
The livestock industry uses ofloxacin, an antibiotic, to prevent several animal diseases; however, the overdose of ofloxacin used in animal farming treatments may appear in food products and cause some adverse human health effects. Hence, there is an immediate need to develop a method suitable for on site large-scale detection of ofloxacin residues in animal-derived foods. This study aimed to prepare a monoclonal antibody with high sensitivity and affinity for ofloxacin by re-synthesizing the ofloxacin hapten and synthesizing the corresponding complete antigen. The IC of the enzyme-linked immunosorbent assay (ic-ELISA) was 0.13 ng/mL, and the detection limit was 0.033 ng/mL. The visual detection limit of the established colloidal gold immunochromatographic test strip, for the visual detection of actual samples, was 1 ng/g. In summary, this work establishes a rapid detection method of ofloxacin residues on the basis of colloidal gold immunochromatography that is suitable for actual detection.
畜牧业使用抗生素氧氟沙星来预防多种动物疾病；然而，动物养殖治疗中使用的氧氟沙星过量可能会出现在食品中，并对人体健康造成一些不良影响。因此，迫切需要开发一种适用于现场大规模检测动物源性食品中氧氟沙星残留的方法。本研究旨在通过重新合成氧氟沙星半抗原并合成相应的完全抗原，制备对氧氟沙星具有高灵敏度和亲和力的单克隆抗体。酶联免疫吸附测定（ic-ELISA）的IC为0.13 ng/mL，检测限为0.033 ng/mL。所建立的胶体金免疫层析试纸条对实际样品进行目视检测的目视检测限为1 ng/g。综上所述，本工作建立了一种基于胶体金免疫层析的氧氟沙星残留快速检测方法，适用于实际检测。
