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超声辅助酶法从[具体来源未给出]中提取多糖：优化、结构表征及对α-突触核蛋白聚集的抑制作用

Ultrasound-Assisted Enzymatic Extraction of Polysaccharides from : Optimization, Structural Characterization, and Inhibition of α-Synuclein Aggregation.

作者信息

Gao Wen, Wang Yang, Lu Fuping, Liu Fufeng

机构信息

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.

出版信息

Foods. 2024 Dec 21;13(24):4150. doi: 10.3390/foods13244150.

DOI:10.3390/foods13244150
PMID:39767092
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11675543/
Abstract

This study optimized ultrasound-assisted enzymatic (UAE) extraction of TMP ( polysaccharide) through response surface methodology. The optimal conditions included complex enzyme comprising 1.15% cellulase, 0.60% pectinase, and 0.95% dispase, with ultrasound for 24 min at 84.5 °C and enzyme hydrolysis at pH 5.0. This process yielded 19.74 ± 0.51% TMP, exceeding traditional hot water extraction by over four times. Fourier transform infrared spectroscopy (FT-IR) confirmed that UAE did not alter the structure of TMP. In vitro experiments indicated that TMP-UAE demonstrated enhanced antioxidant properties. Further purification through DEAE-52 and Sephadex G-100 chromatography resulted in a homogenous polysaccharide fraction (TMP). Characterization indicated that TMP has an average molecular weight of 2.79 × 10 Da, composed of fucose, galactose, glucose and mannose in a 2.00:9.44:86.29:2.28 molar ratio. FT-IR indicated the presence of C-O-C glycosidic bonds and pyranyl-type sugar rings. Scanning electron microscopy displayed loose lamellar structures with small pores. Finally, TMP exhibited therapeutic potential against in Parkinson's disease, including reducing α-synuclein aggregation, protecting dopaminergic neurons, and prolonging lifespan. This study provides an efficient extraction method for TMP and an insight into its neuroprotective effect in PD

摘要

本研究通过响应面法优化了超声辅助酶法（UAE）提取TMP（多糖）的工艺。最佳条件包括复合酶，其中纤维素酶含量为1.15%、果胶酶含量为0.60%、中性蛋白酶含量为0.95%，在84.5℃下超声处理24分钟，酶解pH值为5.0。此工艺得到的TMP产率为19.74±0.51%，比传统热水提取法高出四倍多。傅里叶变换红外光谱（FT-IR）证实UAE未改变TMP的结构。体外实验表明，经UAE处理的TMP具有增强的抗氧化性能。通过DEAE-52和Sephadex G-100柱色谱进一步纯化得到了均一的多糖级分（TMP）。表征显示，TMP的平均分子量为2.79×10 Da，由岩藻糖、半乳糖、葡萄糖和甘露糖以2.00:9.44:86.29:2.28的摩尔比组成。FT-IR表明存在C-O-C糖苷键和吡喃型糖环。扫描电子显微镜显示具有小孔的松散层状结构。最后，TMP在帕金森病中表现出治疗潜力，包括减少α-突触核蛋白聚集、保护多巴胺能神经元和延长寿命。本研究为TMP提供了一种高效提取方法，并深入了解了其在帕金森病中的神经保护作用 。 （注：原文中“average molecular weight of 2.79 × 10 Da”似乎有误，推测可能是“2.79 × 10^x Da”之类的表述，但按原文翻译了。）

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