Che Xiangguo, Kim Hee-June, Jin Xian, Kim Joon-Woo, Park Kyeong-Hyeon, Lim Jeong-Ok, Kyung Hee-Soo, Oh Chang-Wug, Choi Je-Yong
Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
Department of Orthopedic Surgery, Kyungpook National University Hospital, School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
Biomedicines. 2024 Nov 28;12(12):2731. doi: 10.3390/biomedicines12122731.
Bone marrow aspiration concentrate (BMAC) has garnered increasing interest due to its potential for healing musculoskeletal injuries. While the iliac crest remains a common harvest site, the aspiration technique's efficacy in offering the highest yield and prevalence of mesenchymal stem cells (MSCs) is controversial. This study aimed to compare two different techniques of bone marrow aspiration over the anterior iliac crest from a single level versus multiple levels.
Anterior iliac crests were selected in seven adult patients (aged between 31 and 59 years old). Aspiration was achieved using an 11-gauge needle (length: 100 mm; diameter: 2.3 mm) specifically manufactured for bone marrow collection (BD, Becton, Franklin Lakes, NJ, USA) connected to a 10 mL syringe. On one side, 4cc of bone marrow was aspirated at a single level to a depth of 7 cm without changing the needle direction. On the other side, over the same portion of the iliac crest, 1 cc of bone marrow was obtained from multiple levels of different depths during needle retrieval, maintaining a distance of 1 cm and changing the tip direction. The samples were blindly sent to the laboratory without indicating whether they came from an single level or multiple levels. Fluorescence-activated cell sorting (FACS) and osteoblast differentiation were analyzed and compared.
In the FACS analysis, the single level resulted in a higher population of MSCs that were positive for CD105, CD73, and CD90 and negative for CD34, compared to the multiple-level method. In the process of osteoblast differentiation, it was observed that MSCs exhibited more advanced features of enhanced osteoblastic abilities in the single-level method rather than the multiple-level method.
A single-level aspiration technique at the anterior iliac crest may produce a high-quality bone marrow aspirate. This technique may help obtain specific populations of MSCs with the desired characteristics for use in regenerative therapies for musculoskeletal injuries.
骨髓抽吸浓缩物（BMAC）因其在治疗肌肉骨骼损伤方面的潜力而越来越受到关注。虽然髂嵴仍然是常用的采集部位，但抽吸技术在提供最高产量和间充质干细胞（MSCs）患病率方面的功效存在争议。本研究旨在比较从单一层面与多个层面在髂前嵴进行两种不同骨髓抽吸技术的效果。
选取7名成年患者（年龄在31至59岁之间）的髂前嵴。使用专门为骨髓采集制造的11号针头（长度：100毫米；直径：2.3毫米）（美国新泽西州富兰克林湖的BD公司生产）连接到10毫升注射器进行抽吸。在一侧，在不改变针头方向的情况下，在单一层面抽吸4立方厘米骨髓至7厘米深度。在另一侧，在髂嵴的相同部位，在针头回撤过程中从不同深度的多个层面获取1立方厘米骨髓，保持1厘米的间距并改变针尖方向。样本在不表明来自单一层面还是多个层面的情况下被盲目送往实验室。对荧光激活细胞分选（FACS）和成骨细胞分化进行分析和比较。
在FACS分析中，与多层面方法相比，单一层面产生的CD105、CD73和CD90呈阳性且CD34呈阴性的间充质干细胞数量更多。在成骨细胞分化过程中，观察到间充质干细胞在单一层面方法中比多层面方法表现出更高级的成骨能力增强特征。
髂前嵴的单一层面抽吸技术可能产生高质量的骨髓抽吸物。该技术可能有助于获得具有所需特性的特定间充质干细胞群体，用于肌肉骨骼损伤的再生治疗。
