Anitua Eduardo, Muruzabal Francisco, Recalde Sergio, Fernandez-Robredo Patricia, Alkhraisat Mohammad Hamdan
BTI Biotechnology Institute, 01005 Vitoria, Spain.
University Institute for Regenerative Medicine and Oral Implantology-UIRMI (UPV/EHU-Fundación Eduardo Anitua), 01007 Vitoria, Spain.
Medicina (Kaunas). 2024 Dec 10;60(12):2036. doi: 10.3390/medicina60122036.
: Age-related macular degeneration (AMD) is the leading cause of low vision and legal blindness in adults in developed countries. Wet AMD can be successfully treated using vascular endothelial growth factor (VEGF) inhibitors; however, dry AMD currently has no effective treatment. The purpose of this study is to analyze the efficacy of intraocular injection of plasma rich in growth factors (PRGF) in an AMD mouse model induced by intraperitoneal administration of sodium iodate. : Intravitreal application of PRGF (experimental group) and saline (control group) was performed immediately after intraperitoneal injection of sodium iodate. Retinographies were performed at 2 and 7 days after treatment administration. The eyes were retrieved for histological and immunohistological analysis. Statistical analysis was performed to compare the outcomes between the study groups. : In comparison to saline solution, PRGF significantly decreased the depigmentation of the RPE, showing a more reddened retina. PRGF intravitreal treatment significantly reduced the glial fibrillary acidic protein (GFAP) stained processes, suggesting a significant reduction in the risk of scar formation. Moreover, the myofibroblast invasion into the RPE cell layer was significantly reduced in the PRGF-treated group of mice. There was a tendency for better preservation of the photoreceptors in the PRGF group. : Within the limitations of this study, intravitreal injection of PRGF provided significant protection against the degeneration of the photoreceptors and the RPE induced by the systemic administration of NaIO.
年龄相关性黄斑变性（AMD）是发达国家成年人视力低下和法定失明的主要原因。湿性AMD可使用血管内皮生长因子（VEGF）抑制剂成功治疗；然而，干性AMD目前尚无有效治疗方法。本研究的目的是分析眼内注射富含生长因子的血浆（PRGF）在腹腔注射碘酸钠诱导的AMD小鼠模型中的疗效。：在腹腔注射碘酸钠后立即进行玻璃体内注射PRGF（实验组）和生理盐水（对照组）。在给药后2天和7天进行视网膜造影。取出眼睛进行组织学和免疫组织学分析。进行统计分析以比较研究组之间的结果。：与生理盐水相比，PRGF显著减少了视网膜色素上皮（RPE）的色素脱失，视网膜呈现更红的颜色。玻璃体内注射PRGF显著减少了胶质纤维酸性蛋白（GFAP）染色的突起，表明瘢痕形成风险显著降低。此外，在PRGF治疗的小鼠组中，肌成纤维细胞向RPE细胞层的浸润显著减少。PRGF组有更好地保存光感受器的趋势。：在本研究的局限性内，玻璃体内注射PRGF对全身给予NaIO诱导的光感受器和RPE变性提供了显著的保护作用。
