de Deus Ezequiel, Passos Joseane, van Sauers-Muller Alies, Jesus Cristiane, Silva Janisete Gomes, Adaime Ricardo
Instituto Federal do Amapá, Rodovia BR 210 KM 3, s/n, Macapá 68909-398, Amapá, Brazil.
Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá, Programa de Pós-Graduação em Biodiversidade Tropical, Rodovia JK, Km 4, Macapá 68902-280, Amapá, Brazil.
Insects. 2024 Nov 30;15(12):949. doi: 10.3390/insects15120949.
The carambola fruit fly, Drew & Hancock, is native to Southeast Asia, infests about 150 plant species, and is considered a quarantine pest insect in several regions of the world. has invaded Suriname, French Guyana, and northern Brazil. In Brazil, it was first recorded in 1996 and has been restricted to the states of Amapá and Roraima due to official control efforts of the Ministry of Agriculture and Food Supply (Ministério da Agricultura e Pecuária-MAPA). This is the first study to estimate the genetic structure and diversity of South American populations of . A total of 116 individuals from 11 localities in Brazil and 7 localities in Suriname were analyzed. Additional sequences available at GenBank from Indonesia (Lampung) and Thailand (San Pa Tong and Muang District) were also used in the analysis. We sequenced a fragment of the mitochondrial gene cytochrome oxidase subunit I. A total of 35 haplotypes were found. Haplotypes from Indonesia were closest to the haplotypes from South America, separated only by a few mutational steps. This suggests that Indonesia is the likely source for the introduction of into South America. The Southeast Asian populations appeared as the most ancestral group in the phylogenetic trees. The high similarity and sharing of several haplotypes among populations within South America indicate a lack of genetic structure. The mismatch distribution and neutrality tests suggest that South American populations have undergone a rapid growth and expansion following a single founder event. The low genetic diversity and the population expansion evidenced by the neutrality tests lend support to the hypothesis of a recent introduction of a single lineage of the carambola fruit fly into South America.
杨桃实蝇（Drew & Hancock）原产于东南亚，侵害约150种植物，在世界多个地区被视为检疫性害虫。它已入侵苏里南、法属圭亚那和巴西北部。在巴西，它于1996年首次被记录，由于农业和粮食供应部（Ministério da Agricultura e Pecuária - MAPA）的官方控制措施，其分布仅限于阿马帕州和罗赖马州。这是首次对南美洲杨桃实蝇种群的遗传结构和多样性进行估计的研究。对来自巴西11个地点和苏里南7个地点的总共116个个体进行了分析。分析中还使用了来自印度尼西亚（楠榜）和泰国（清迈府讪巴东和清迈市）的GenBank上可获得的其他序列。我们对线粒体基因细胞色素氧化酶亚基I的一个片段进行了测序。共发现35个单倍型。来自印度尼西亚的单倍型与来自南美洲的单倍型最为接近，仅相隔几个突变步骤。这表明印度尼西亚可能是杨桃实蝇引入南美洲的来源地。东南亚种群在系统发育树中表现为最原始的群体。南美洲各群体之间几种单倍型的高度相似性和共享表明缺乏遗传结构。失配分布和中性检验表明，南美洲种群在一次单一奠基者事件后经历了快速增长和扩张。中性检验所证明的低遗传多样性和种群扩张支持了最近将杨桃实蝇的单一谱系引入南美洲的假说。
