College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China.
Food Chem Toxicol. 2012 Nov;50(11):4016-22. doi: 10.1016/j.fct.2012.08.007. Epub 2012 Aug 10.
Eclipta prostrata L., (Asteraceae), is used in China for both food and medicine purposes. This research is concerned with the isolation and purification of phytochemical constituents from the aerial parts of E. prostrata, using gradient solvent fractionation, macroporous resin, silica gel, Sephadex LH-20 and ODS columns, and TLC analyses. Four fractions (water, 30% ethanol, 60% ethanol and 90% ethanol) were obtained. Four compounds, wedelolactone (I), eclalbasaponin I (II), luteolin (III) and luteolin-7-O-glucoside (IV) were purified and their structures were identified by the interpretation of spectroscopic analyses including MS, (1)H and (13)C NMR. Antitumor activities of extracts (total fraction), four fractions and the isolated compounds were assessed using hepatoma cell smmc-7721 as an in vitro assay system. The 30% ethanol fraction and eclalbasaponin I dose-dependently inhibited the proliferation of hepatoma cell smmc-7721 with IC(50) values of 74.2399 and 111.1703 μg/ml, respectively, more strongly compared with 5-fluorouracil positive control group with the IC(50) value of 195.3131 μg/ml. Antitumor activities of other fractions and compounds were lower than positive control. These results suggested that some specific compounds or extracts from E. prostrata are potential sources of natural anti-tumor materials and worthy of further study.
垂盆草（Asteraceae），在中国既作为食品又作为药品使用。本研究关注的是通过梯度溶剂分级、大孔树脂、硅胶、Sephadex LH-20 和 ODS 柱和 TLC 分析，从垂盆草的地上部分分离和纯化植物化学成分。得到四个馏分（水、30%乙醇、60%乙醇和 90%乙醇）。通过解析包括 MS、（1）H 和（13）C NMR 在内的光谱分析，分离和鉴定了四种化合物，即齐墩果酸内酯（I）、垂盆草皂苷 I（II）、木犀草素（III）和木犀草素-7-O-葡萄糖苷（IV）。采用体外测定系统肝癌细胞 smmc-7721 评估提取物（总馏分）、四个馏分和分离化合物的抗肿瘤活性。30%乙醇馏分和垂盆草皂苷 I 剂量依赖性地抑制肝癌细胞 smmc-7721 的增殖，IC（50）值分别为 74.2399 和 111.1703μg/ml，比阳性对照 5-氟尿嘧啶（IC（50）值为 195.3131μg/ml）更强。其他馏分和化合物的抗肿瘤活性低于阳性对照。这些结果表明，垂盆草中的一些特定化合物或提取物可能是天然抗肿瘤物质的潜在来源，值得进一步研究。
