Lee Hwaryeong, Youn Isoo, Noh Sang Gyun, Kim Hyun Woo, Song Eunhye, Nam Sang-Jip, Chung Hae Young, Seo Eun Kyoung
Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul 03760, Republic of Korea.
Department of Pharmacy, College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea.
Molecules. 2024 Dec 20;29(24):6009. doi: 10.3390/molecules29246009.
Libosch., which belongs to the Orobanchaceae family, is a perennial herb found in China, Japan, and Korea. In traditional medicine, it is used to cool the body, improve water metabolism in the kidney, and provide protection from metabolic diseases such as type 2 diabetes mellitus (T2DM) and obesity. In this study, three new compounds were isolated from the roots of , along with eighteen known compounds. Structure elucidation was performed with spectroscopic analyses including nuclear magnetic resonance (NMR) and circular dichroism (CD) spectroscopy. As the AMP-activated protein kinase (AMPK) signaling pathway is reportedly related to metabolic diseases, AMPK activation studies were conducted using in silico simulations and in vitro assays. Among the isolated compounds, showed a potential as an AMPK activator in both in silico simulations and in vitro experiments. Our findings expand the chemical profiles of the plant and suggest that one newly found compound () activates AMPK.
列当属于列当科，是一种在中国、日本和韩国均有发现的多年生草本植物。在传统医学中，它被用于清热去火、改善肾脏的水代谢，并预防诸如2型糖尿病（T2DM）和肥胖症等代谢性疾病。在本研究中，从列当的根部分离出了三种新化合物以及十八种已知化合物。通过包括核磁共振（NMR）和圆二色（CD）光谱在内的光谱分析进行了结构解析。由于据报道AMP激活的蛋白激酶（AMPK）信号通路与代谢性疾病有关，因此使用计算机模拟和体外试验进行了AMPK激活研究。在所分离出的化合物中，[具体化合物名称未给出]在计算机模拟和体外实验中均显示出作为AMPK激活剂的潜力。我们的研究结果扩展了列当这种植物的化学特征谱，并表明一种新发现的化合物（[具体化合物名称未给出]）可激活AMPK。
