Costa Martim, van der Geer Liselotte, Joaquim Miguel, Hammarström B, Tanriverdi S, Joensson H N, Wiklund M, Russom A
Science for Life Laboratory, Department of Protein Science, Division of Nanobiotechnology, KTH Royal Institute of Technology, 171 65 Solna, Sweden.
Departamento de Bioengenharia, Instituto Superior Técnico, 1049-001 Lisboa, Portugal.
Micromachines (Basel). 2024 Dec 11;15(12):1487. doi: 10.3390/mi15121487.
Micro- and nanoplastics have become increasingly relevant as contaminants to be monitored due to their potential health effects and environmental impact. Nanoplastics, in particular, have been shown to be difficult to detect in drinking water, requiring new capture technologies. In this work, we applied the acoustofluidic seed particle method to capture nanoplastics in an optimized, tilted grid of silica clusters even at the high flow rate of 5 mL/min. Moreover, we achieved, using this technique, the enrichment of nanoparticles ranging from 500 nm to 25 nm as a first in the field. We employed fluorescence to observe the enrichment profiles according to size, using a washing buffer flow at 0.5 mL/min, highlighting the size-dependent nature of the silica seed particle release of various sizes of nanoparticles. These results highlight the versatility of acoustic trapping for a wide range of nanoplastic particles and allow further study into the complex dynamics of the seed particle method at these size ranges. Moreover, with reproducible size-dependent washing curves, we provide a new window into the rate of nanoplastic escape in high-capacity acoustic traps, relevant to both environmental and biomedical applications.
由于微塑料和纳米塑料具有潜在的健康影响和环境影响，它们作为需要监测的污染物变得越来越重要。特别是纳米塑料，已被证明在饮用水中难以检测，需要新的捕获技术。在这项工作中，我们应用声流体种子颗粒法在优化的倾斜二氧化硅簇网格中捕获纳米塑料，即使在5毫升/分钟的高流速下也能实现。此外，我们利用这项技术首次在该领域实现了对500纳米至25纳米范围内纳米颗粒的富集。我们使用荧光来观察不同尺寸纳米颗粒的富集情况，以0.5毫升/分钟的流速使用洗涤缓冲液，突出了不同尺寸纳米颗粒二氧化硅种子颗粒释放的尺寸依赖性。这些结果突出了声捕获对广泛纳米塑料颗粒的通用性，并允许对这些尺寸范围内种子颗粒法的复杂动力学进行进一步研究。此外，通过可重复的尺寸依赖性洗涤曲线，我们为高容量声阱中纳米塑料逃逸速率提供了一个新窗口，这与环境和生物医学应用都相关。
