Manyi-Loh Christy Echakachi, Okoh Anthony Ifeanyin, Lues Ryk
Centre of Applied Food Sustainability and Biotechnology (CAFSaB), Central University of Technology, Bloemfontein 9301, South Africa.
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice 5700, South Africa.
Microorganisms. 2023 Mar 11;11(3):725. doi: 10.3390/microorganisms11030725.
is a zoonotic foodborne pathogen with inherent adaptability to tolerate environmental and physiological stresses, thereby causing severe disease outbreaks. Antibiotic resistant foodborne pathogens are a challenge to the food industry. A total of 18 samples were pooled from a bio-digester co-digesting swine manure/pinewood sawdust, and evaluated for the occurrence of bacterium plus total viable counts using the spread plate method. The recovered bacterial isolates were presumptively identified by growth on selective medium and confirmed by biochemical characterisation, leading to the isolation of 43 . . The isolates were characterized based on their susceptibility to antibiotics via the Kirby-Bauer disc diffusion technique against a panel of 14 antibiotics. Equally, the multiple antibiotic resistance (MAR) index was calculated, and MAR phenotypes generated. The bacterial counts were between 10 and10 cfu/mL. Complete susceptibility (100%) was demonstrated to ampicillin, gentamicin and sulfamethoxazole, which are the drugs of choice in the treatment of listeriosis. In addition, intermediate sensitivity occurred at 25.58% to cefotaxime, and the highest resistance (51.16%) was exhibited against nalidixic acid. The MAR index ranged from 0 to 0.71. Overall, 41.86% of the Listeria isolates displayed multidrug resistance, with 18 different MAR phenotypes, demonstrating CIP, E, C, TET, AUG, S, CTX, NA, AML, NI as the greatest MAR phenotype. It can be concluded that the isolates yielding MAR > 0.2 originated from the farm, where antibiotics had been in routine use. Therefore, strict monitoring of antibiotics use in the farm is crucial to mitigate further increase in antibiotic resistance amongst these bacterial isolates.
是一种人畜共患食源性病原体，具有内在的适应性以耐受环境和生理压力，从而引发严重的疾病暴发。抗生素耐药性食源性病原体对食品行业构成挑战。从共同消化猪粪/松木锯末的生物消化器中总共采集了18个样本，并使用平板涂布法评估细菌的存在情况以及总活菌数。通过在选择性培养基上生长对回收的细菌分离株进行初步鉴定，并通过生化特征进行确认，从而分离出43株……通过Kirby-Bauer纸片扩散技术针对一组14种抗生素，根据分离株对抗生素的敏感性对其进行表征。同样，计算多重抗生素耐药性（MAR）指数，并生成MAR表型。细菌计数在10⁵至10⁷cfu/mL之间。对氨苄青霉素、庆大霉素和磺胺甲恶唑表现出完全敏感性（100%），这些是治疗李斯特菌病的首选药物。此外，对头孢噻肟的中度敏感性发生率为25.58%，对萘啶酸表现出最高耐药性（51.16%）。MAR指数范围为0至0.71。总体而言，41.86%的李斯特菌分离株表现出多重耐药性，有18种不同的MAR表型，表明CIP、E、C、TET、AUG、S、CTX、NA、AML、NI为最主要的MAR表型。可以得出结论，MAR>0.2的分离株源自农场，那里常规使用抗生素。因此，严格监测农场中抗生素的使用对于减轻这些细菌分离株中抗生素耐药性的进一步增加至关重要。
