Chtourou Khouloud, Salazar Juan Alfonso, Ortuño-Hernández Germán, Mezghani Najla, Trifi-Farah Neila, Martínez-Gómez Pedro, Krichen Lamia
LR99ES12, Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, Tunis 2092, Tunisia.
Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura-Consejo Superior de Investigaciones Científicas (CEBAS-CSIC), Campus Universitario Espinardo, E-30100 Murcia, Spain.
Plants (Basel). 2024 Dec 20;13(24):3563. doi: 10.3390/plants13243563.
Assessing and determining genetic diversity in rose species is a crucial step for conservation efforts, the establishment of a core collection, and the development of new varieties. This study represents the first investigation of genetic diversity among various rose species at different ploidy levels in Tunisia, with the aim of elucidating the genetic structure of the genus. It encompasses both spontaneous and cultivated accessions, featuring local and introduced species recognized for their adaptability, ornamental value, and fragrance. A total of 114 accessions representing eight rose species were collected. Significant genetic diversity was assessed using seven SSR markers, yielding an average of 21 alleles per locus and a PIC value ranging from 0.882 to 0.941. The results identified 343 phenotypic alleles across the seven primers, with 72 for the primer RhE2b (LG6), 55 for H10D03 (LG7), and 54 for RhB303 (LG2). One key finding was that most perfumed rose accessions ( Mill. and L.) are distinct from the other rose accessions, indicating a unique genetic pool for these roses. Another important finding was that the Tunisian accessions of 'Rose of Ariana' were closely related to accessions, commonly known as the 'Rose of May' from Grasse, France. To clarify the phylogeny of this species or subspecies, further molecular studies are warranted. Additionally, nomenclature confusion was identified between L. and L. in the northwestern region of Tunisia, indicating that all wild accessions correspond to .
评估和确定玫瑰品种的遗传多样性是保护工作、建立核心种质库以及培育新品种的关键步骤。本研究首次对突尼斯不同倍性水平的各种玫瑰品种的遗传多样性进行了调查，旨在阐明该属的遗传结构。研究涵盖了自发和栽培品种，包括因适应性、观赏价值和香气而闻名的本地和引进品种。总共收集了代表8个玫瑰品种的114份种质。使用7个SSR标记评估了显著的遗传多样性，每个位点平均产生21个等位基因，PIC值在0.882至0.941之间。结果在7个引物中鉴定出343个表型等位基因，其中引物RhE2b（LG6）有72个，H10D03（LG7）有55个，RhB303（LG2）有54个。一个关键发现是，大多数香水玫瑰品种（百叶蔷薇和突厥蔷薇）与其他玫瑰品种不同，表明这些玫瑰有独特的基因库。另一个重要发现是，突尼斯的“阿丽亚娜玫瑰”品种与通常被称为法国格拉斯的“五月玫瑰”的品种密切相关。为了阐明该物种或亚种的系统发育，有必要进行进一步的分子研究。此外，在突尼斯西北部地区，百叶蔷薇和突厥蔷薇之间存在命名混淆，这表明所有野生种质都对应于百叶蔷薇。
