玉米条纹病毒：单壳和双联体衣壳结构

Maize Streak Virus: Single and Gemini Capsid Architecture.

作者信息

Bennett Antonette, Hull Joshua A, Mietzsch Mario, Bhattacharya Nilakshee, Chipman Paul, McKenna Robert

机构信息

Department of Biochemistry and Molecular Biology, College of Medicine Center for Structural Biology, McKnight Brain Institute, University of Florida, Gainesville, FL 32610-0245, USA.

Biological Science Imaging Facility (BSIR), Department of Biology, Florida State University, 89 Chieftain Way, Tallahassee, FL 32306-4370, USA.

出版信息

Viruses. 2024 Nov 29;16(12):1861. doi: 10.3390/v16121861.

DOI:10.3390/v16121861

PMID:39772173

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11680415/

Abstract

are ssDNA plant viruses whose control has both economical and agricultural importance. Their capsids assemble into two distinct architectural forms: (i) a T = 1 icosahedral and (ii) a unique twinned quasi-isometric capsid. Described here are the high-resolution structures of both forms of the maize streak virus using cryo-EM. A comparison of these two forms provides details of the coat protein (CP) and CP-CP and CP-genome interactions that govern the assembly of the architecture of the capsids. Comparative analysis of other representative members of reveals structural conservation of 60-95% compared to a sequence similarity of 21-30%. This study provides a structural atlas of these plant pathogens and suggests possible antiviral-targetable regions of these capsids.

摘要

是单链DNA植物病毒，其控制具有经济和农业重要性。它们的衣壳组装成两种不同的结构形式：（i）T = 1二十面体，（ii）独特的孪生准等距衣壳。本文使用冷冻电镜描述了玉米条纹病毒两种形式的高分辨率结构。这两种形式的比较提供了衣壳蛋白（CP）以及控制衣壳结构组装的CP-CP和CP-基因组相互作用的细节。对其他代表性成员的比较分析表明，与21-30%的序列相似性相比，结构保守性为60-95%。这项研究提供了这些植物病原体的结构图谱，并指出了这些衣壳可能的抗病毒靶向区域。