Fabiansson S, Rutegård A
Acta Vet Scand. 1979;20(4):477-91. doi: 10.1186/BF03546575.
Biological methods in current use for the detection of antibiotic residues in slaughter animals are reviewed. A modified method is suggested in which the conditions for the control have been standardized. By the use of a semi-defined medium, the batch-to-batch variations are minimized. In order to facilitate the detection of sulfonamides the medium is supplemented with trimethoprim. The standardized conditions included the use of a sporulating organism, Bacillus subtilis, an inoculum size of 0.5 × 10 spores per ml medium, and 5 ml medium of pH 6.0 per plate. A preincubation-diffusion time of 1 h in room temperature is recommended before incubation. The modified method was compared with the currently prescribed Swedish method. The new method was easier to perform and showed a more uniform sensitivity to most of the antibiotics used.
本文综述了目前用于检测屠宰动物抗生素残留的生物学方法。提出了一种改进方法，其中对照条件已标准化。通过使用半限定培养基，批次间差异最小化。为便于检测磺胺类药物，培养基中添加了甲氧苄啶。标准化条件包括使用产孢生物体枯草芽孢杆菌、每毫升培养基接种量为0.5×10个孢子以及每平板使用5毫升pH 6.0的培养基。建议在培养前于室温下预孵育扩散1小时。将改进后的方法与瑞典目前规定的方法进行了比较。新方法操作更简便，对大多数使用的抗生素显示出更均匀的敏感性。
