一种用于检测食品中磺胺类药物残留的改良细菌学方法。
An improved bacteriological method for the detection of sulfonamide residues in food.
作者信息
Gudding R
出版信息
Acta Vet Scand. 1976;17(4):458-64. doi: 10.1186/BF03547901.
Abstract
The addition of trimethoprim to Mueller-Hinton medium was found to improve the sensitivity of test bacteria towards sulfonamides by 20—50 times. Depending on the test bacteria used, the optimal concentrations of trimethoprim added to the medium are 1 μg per ml (Micrococcus luteus), 0.25 μg per ml (Bacillus stearothermophilus var. calidolactis) and 0.1 μg per ml (Bacillus megaterium). Using Micrococcus luteus or Bacillus megaterium a concentration of 0.25 μg sulfanilamide per ml may be detected, and 0.1 μg per ml may be detected when Bacillus stearothermophilus var. calidolactis is used.
摘要
已发现向穆勒-欣顿培养基中添加甲氧苄啶可使测试细菌对磺胺类药物的敏感性提高20至50倍。根据所使用的测试细菌，添加到培养基中的甲氧苄啶的最佳浓度分别为每毫升1微克（藤黄微球菌）、每毫升0.25微克（嗜热脂肪芽孢杆菌嗜热变种）和每毫升0.1微克（巨大芽孢杆菌）。使用藤黄微球菌或巨大芽孢杆菌时，每毫升可检测到0.25微克磺胺，而使用嗜热脂肪芽孢杆菌嗜热变种时，每毫升可检测到0.1微克。
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本文引用的文献
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