Guan Yapeng, Wang Jun, Liu Meng, Lu Xinli
Department of Food and Drug Engineering, Shijiazhuang College of Applied Technology, Shijiazhuang, China.
College of Life Science, Cangzhou Normal University, Cangzhou, China.
AIDS Res Hum Retroviruses. 2025 Apr;41(4):220-224. doi: 10.1089/aid.2024.0104. Epub 2025 Jan 8.
Acquired immune deficiency syndrome caused by human immunodeficiency virus (HIV) is a serious infectious disease because of its' high genetic variability. Nowadays, homosexual contact has become the most predominant transmission route in Hebei province, China, leading to the emergence of novel HIV-1 recombinant forms. The neighbor-joining (N-J) phylogenetic trees were constructed using MEGA 6.0 in order to identify the subtypes of H22063 and H22144. Recombination breakpoints were identified using online resources jpHMM, RIP 3.0, and Simplot 3.5.1. In this study, we identified two novel HIV-1 unique recombinant forms (URFs) _0107 from three men who have sex with men in Hebei province, including H22063 and H22144. The near full-length genome analysis showed H22063 has seven gene recombination sub-regions, including three subtype CRF07_BC gene fragments inserted into the CRF01_AE backbone. H22144 has nine gene recombination sub-regions, including four subtype CRF07_BC gene fragments inserted into the CRF01_AE backbone. This study confirms the emergence of novel recombinant forms and suggests we should strengthen the monitoring of novel HIV recombinant forms in order to deal with the complex HIV-1 epidemiological trend in Hebei province, China.
由人类免疫缺陷病毒（HIV）引起的获得性免疫缺陷综合征是一种严重的传染病，因其具有高度的基因变异性。如今，在中国河北省，同性恋接触已成为最主要的传播途径，导致出现了新型HIV-1重组形式。使用MEGA 6.0构建邻接法（N-J）系统发育树，以鉴定H22063和H22144的亚型。利用在线资源jpHMM、RIP 3.0和Simplot 3.5.1鉴定重组断点。在本研究中，我们从河北省三名男男性行为者中鉴定出两种新型HIV-1独特重组形式（URF）_0107，包括H22063和H22144。近乎全长的基因组分析表明，H22063有七个基因重组子区域，包括三个插入CRF01_AE主干的CRF07_BC亚型基因片段。H22144有九个基因重组子区域，包括四个插入CRF01_AE主干的CRF07_BC亚型基因片段。本研究证实了新型重组形式的出现，并表明我们应加强对新型HIV重组形式的监测，以应对中国河北省复杂的HIV-1流行病学趋势。
