Clinical Laboratory, The People's Hospital of Baoding, Baoding, China.
AIDS Res Hum Retroviruses. 2024 Jul;40(7):449-453. doi: 10.1089/AID.2023.0119. Epub 2024 Mar 22.
To analyze the genetic structure and recombination characteristics of a newly discovered HIV-1 unique recombinant form (URF) isolated in Hebei Province, China, viral RNA was extracted from the plasma sample of the infected individual and reverse transcribed to cDNA. Two overlapping segments of the HIV-1 genome were amplified using a near-endpoint dilution method. Recombinant breakpoints were determined using RIP, jpHMM, and SimPlot 3.5.1 software. MEGA 6.0 software was used to construct a neighbor-joining phylogenetic tree. The near full-length genome sequence (8,862 bp) of a recombinant of CRF01_AE/CRF07_BC was obtained. The genome comprised at least seven overlapping segments, including four CRF01_AE and three CRF07_BC segments, with CRF01_AE as the backbone. A URF virus between CRF01_AE and CRF07_BC was amplified and characterized in this study. Parental viruses were homologous with HIV-1 strains prevalent among men who have sex with men in northern China and may originate from sexual transmission of local HIV-1 strains in Hebei Province.
为分析在中国河北省分离到的一种新的 HIV-1 独特重组形式（URF）的遗传结构和重组特征，从感染者的血浆样本中提取病毒 RNA，反转录为 cDNA。使用近端点稀释法扩增 HIV-1 基因组的两个重叠片段。使用 RIP、jpHMM 和 SimPlot 3.5.1 软件确定重组断点。使用 MEGA 6.0 软件构建邻接聚类系统发生树。获得了一个重组的 CRF01_AE/CRF07_BC 的近全长基因组序列（8862bp）。该基因组至少包括七个重叠片段，包括四个 CRF01_AE 和三个 CRF07_BC 片段，以 CRF01_AE 为骨架。在这项研究中扩增并表征了 CRF01_AE 和 CRF07_BC 之间的 URF 病毒。亲代病毒与中国北方男男性行为者中流行的 HIV-1 株同源，可能源自河北省当地 HIV-1 株的性传播。
