阴离子通量对线粒体氧化磷酸化的影响。

Influence of anion flux upon mitochondrial oxidative phosphorylation.

作者信息

Howland J L, Barstow J, Mlodinow S G

出版信息

Biochem Biophys Res Commun. 1985 Mar 15;127(2):473-6. doi: 10.1016/s0006-291x(85)80184-4.

Abstract

We have measured mitochondrial ATP synthesis during passive anion influx and find that influx of phosphate leads to diminished efficiency (as reflected in the ATP:0 ratio) whereas influx of acetate produced enhanced efficiency. The anions, sulfate, propionate, and thiocyanate, are without influence on the ATP:0 ratio. It is likely that the opposite effects of phosphate and acetate on the ATP:0 ratio reflect phosphate-acetate exchange, and that acetate influx produces its positive effect on ATP synthesis by promoting phosphate efflux. Thus, phosphate efflux may be associated with increased, and phosphate influx, with decreased energy conservation.

摘要

我们测量了被动阴离子内流过程中的线粒体ATP合成，发现磷酸盐内流会导致效率降低（如ATP:O比率所示），而乙酸盐内流则提高了效率。阴离子、硫酸盐、丙酸盐和硫氰酸盐对ATP:O比率没有影响。磷酸盐和乙酸盐对ATP:O比率的相反影响可能反映了磷酸盐 - 乙酸盐交换，并且乙酸盐内流通过促进磷酸盐外流对ATP合成产生积极影响。因此，磷酸盐外流可能与能量保存增加有关，而磷酸盐内流则与能量保存减少有关。