Son Haelim, Mun Wonsik, Mitchell Robert J, Cho Gyu-Sung, Moon Ja-Young, Lee Kyoung
Department of Bio Health Science, Changwon National University, Changwon, Gyeongnam 51140, Republic of Korea.
School of Life Sciences, Department of Biological Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.
Int J Syst Evol Microbiol. 2025 Jan;75(1). doi: 10.1099/ijsem.0.006617.
Five pink-pigmented bacterial strains, isolated from human skin and classified within the genus , were examined. Among them, four were identified as , while strain OT10 was deemed to be a potential novel species. Strain OT10 exhibited characteristics, such as Gram-stain-negative, oxidase positive, motile, strictly aerobic and rod shaped. The cells had multiple flagella at one end, arranged in a lophotrichous pattern. The predominant cellular fatty acids in OT10 were C ω7c/C ω6c and C 2OH; ubiquinone (Q)-10 was identified as the sole quinone. Major polar lipids included phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine and two aminolipids. The G+C content of the genome was determined to be 72.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarities revealed that strain OT10 is closely related to subsp. ATCC 49956 (97.7%), subsp. ATCC BAA-691 (97.7%) and ATCC BAA-692 (97.5%). For the comparative genomic analyses, whole-genome sequencing was also conducted for strain OT10. Considering the chemotaxonomic, genotypic and phenotypic features, as well as the low average nucleotide identity and digital DNA-DNA hybridization values compared to its closest phylogenomic neighbours, OT10 is proposed to be a novel species named sp. nov., with OT10 designated as the type strain (=KCTC 92087 =JCM 34968).
对从人皮肤分离并归类于该属的五株粉红色色素细菌菌株进行了检测。其中，四株被鉴定为，而菌株OT10被认为是一个潜在的新物种。菌株OT10表现出革兰氏染色阴性、氧化酶阳性、运动性、严格需氧和杆状等特征。细胞一端有多个鞭毛，呈丛生鞭毛排列模式。OT10中主要的细胞脂肪酸为C ω7c/C ω6c和C 2OH；泛醌（Q）-10被鉴定为唯一的醌。主要极性脂质包括磷脂酰甘油、磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰胆碱和两种氨基脂质。基因组的G+C含量测定为72.6 mol%。基于16S rRNA基因序列相似性的系统发育分析表明，菌株OT10与亚种ATCC 49956（97.7%）、亚种ATCC BAA-691（97.7%）和ATCC BAA-692（97.5%）密切相关。为了进行比较基因组分析，还对菌株OT10进行了全基因组测序。考虑到化学分类、基因型和表型特征，以及与其最接近的系统发育邻居相比低的平均核苷酸同一性和数字DNA-DNA杂交值，OT10被提议为一个新物种，命名为新种，OT10被指定为模式菌株（=KCTC 92087 =JCM 34968）。
