Choi Minseok, Chae Yeon, Park Jayeon, Lee Yelim, Park Kyung-Mee, Jeong Dong-Hyuk, Kang Byeong-Teck, Yun Taesik, Kim Hakhyun
Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, South Korea.
Laboratory of Surgery/Ophthalmology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, South Korea.
Vet Med Sci. 2025 Jan;11(1):e70166. doi: 10.1002/vms3.70166.
Studies are currently being conducted on rabbits requiring serial glucose monitoring. The FreeStyle Libre 2 (FSL2), a serial glucose monitoring device, has been validated in humans, dogs and cats, but not in rabbits.
This study aimed to evaluate the accuracy of the FSL2 in rabbits.
Six healthy rabbits were used in this study. Interstitial glucose (IG) was measured using the FSL2, and blood glucose (BG) was measured using a portable blood glucose meter (PBGM); their results were compared with those from a clinical chemistry analyser. For the first 3 h, IG and BG were measured at 1-h intervals. Subsequently, they were measured every 8 h over a 48-h period. Regular insulin 0.2 U/kg was then administered to the rabbits, and IG and BG were measured every 15 min over a 90-min period.
Before insulin treatment, no measurements fell within the hypoglycaemic range (BG < 100 mg/dL). In the euglycaemic range (BG ≥ 100 mg/dL), the PBGM and FSL2 showed 85.7% and 23.8% accuracies, respectively. After insulin treatment, the PBGM showed 95.5% and 81.3% accuracies in the hypoglycaemic and euglycaemic ranges, respectively. The FSL2 showed 68.1% and 37.5% accuracies in the hypoglycaemic and euglycaemic ranges, respectively. Parkes consensus error grid analysis showed that the PBGM and FSL2 had 100% agreement for Zones A (no effect on clinical action) and B (altered clinical action unlikely to affect outcome) in rabbits with and without insulin treatment.
There was limited agreement between the FSL2 and reference standard BG measurements. However, the FSL2 allows clinically acceptable identification of hypoglycaemic states in rabbits.
目前正在对需要连续血糖监测的兔子进行研究。FreeStyle Libre 2（FSL2）是一种连续血糖监测设备，已在人类、犬类和猫类中得到验证，但尚未在兔子中得到验证。
本研究旨在评估FSL2在兔子中的准确性。
本研究使用了6只健康兔子。使用FSL2测量间质葡萄糖（IG），使用便携式血糖仪（PBGM）测量血糖（BG）；将它们的结果与临床化学分析仪的结果进行比较。在最初的3小时内，每隔1小时测量一次IG和BG。随后，在48小时内每8小时测量一次。然后给兔子注射0.2 U/kg的常规胰岛素，并在90分钟内每隔15分钟测量一次IG和BG。
在胰岛素治疗前，没有测量值落在低血糖范围内（BG < 100 mg/dL）。在血糖正常范围内（BG≥100 mg/dL），PBGM和FSL2的准确率分别为85.7%和23.8%。胰岛素治疗后，PBGM在低血糖和血糖正常范围内的准确率分别为95.5%和81.3%。FSL2在低血糖和血糖正常范围内的准确率分别为68.1%和37.5%。Parkes共识误差网格分析表明，在有或没有胰岛素治疗的兔子中，PBGM和FSL2在A区（对临床行动无影响）和B区（临床行动改变不太可能影响结果）的一致性为100%。
FSL与参考标准BG测量之间的一致性有限。然而，FSL2能够在临床上可接受地识别兔子的低血糖状态。
