Wang Yue, He Xinni, Qian Zijin, Li Shuxian, Jing Muzi, Li Xuexia, Shen Wenjia, Xue Shaoshuai, Li Hong, Chen Lian
College of Life Sciences, Nanjing Forestry University, Nanjing, China.
College of Life Sciences, Nanjing Normal University, Nanjing, China.
Integr Zool. 2025 Jan 10. doi: 10.1111/1749-4877.12942.
Pomacea canaliculata is recognized as a globally invasive aquatic species. Analyses of intestinal microbiota, dietary composition, and metabolism of invasive species can enhance our understanding of their feeding strategies and physiological adaptation strategies to the environment. Intestinal content samples were collected from P. canaliculata inhabiting three distinct environments including a pond, a river, and a ditch. These samples were subjected to 16S rRNA gene sequencing analysis and multiple metabarcoding analyses, including eukaryotic 18S rRNA, mitochondrial cytochrome c oxidase I (COI), and chloroplast rbcL genes. In addition, metabolomics analysis was conducted on the intestinal content samples to investigate metabolic change. The highest dietary diversity in P. canaliculata was observed in the ditch, and females exhibited a higher dietary diversity than males in the pond. The 18S rRNA gene has a high potential for identifying the dietary components of omnivorous species. The intestinal microbiota of P. canaliculata from different habitats displayed significant variations, attributed to differences in food resources and other environmental factors. Bacteria in the aquatic environment had minimal impact on the intestinal microbiota of P. canaliculata. Overall, P. canaliculata exhibited adaptive changes in physiological characteristics across different habitats, including alterations in diet, which, in turn, influence microbiota and metabolic pathways such as amino acid biosynthesis in the intestine. The present study investigated the physiological mechanisms that enable P. canaliculata to adapt to diverse habitats, considering various factors including diet, which is important for comprehending its invasive potential and the subsequent threats it poses to aquatic ecosystems.
福寿螺被公认为是一种全球入侵性水生生物。对入侵物种的肠道微生物群、饮食组成和代谢进行分析，有助于我们了解它们的觅食策略以及对环境的生理适应策略。从栖息于池塘、河流和沟渠这三种不同环境中的福寿螺采集肠道内容物样本。这些样本进行了16S rRNA基因测序分析以及多种元条形码分析，包括真核生物18S rRNA、线粒体细胞色素c氧化酶I（COI）和叶绿体rbcL基因。此外，对肠道内容物样本进行了代谢组学分析，以研究代谢变化。在沟渠中观察到福寿螺的饮食多样性最高，在池塘中雌性福寿螺的饮食多样性高于雄性。18S rRNA基因在识别杂食性物种的饮食成分方面具有很高的潜力。来自不同栖息地的福寿螺肠道微生物群表现出显著差异，这归因于食物资源和其他环境因素的不同。水生环境中的细菌对福寿螺肠道微生物群的影响最小。总体而言，福寿螺在不同栖息地的生理特征表现出适应性变化，包括饮食变化，而饮食变化又会影响微生物群和代谢途径，如肠道中的氨基酸生物合成。本研究探讨了福寿螺适应不同栖息地的生理机制，考虑了包括饮食在内的各种因素，这对于理解其入侵潜力以及随后对水生生态系统构成的威胁具有重要意义。
