Meng Yan, Hu Xi, Jiang Nan, Fan Yuding, Li Yiqun, Xue Mingyang, Xu Chen, Liu Wenzhi, Zhou Yong
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Animals (Basel). 2025 Jan 3;15(1):96. doi: 10.3390/ani15010096.
is an interferon-stimulated gene (ISG) that plays an important role in the congenital antiviral immunity of vertebrates. In this study, the common carp () gene is characterized, and we determine whether it has the ability to inhibit spring viremia of carp virus (SVCV) replication in EPC cells. The results showed that the full-length cDNA of the gene was 1044 bp and it encoded 348 amino acids. The cc-viperin sequence contained a leucine zipper in the N-terminal, a CxxxCxxC motif in the SAM domain, and a conservative C-terminus. The gene's nucleotide and amino acid sequence alignment revealed that displayed relatively high sequence identity compared with other species. Phylogenetic analysis displayed the close relation of cc-viperin with and . Subcellular localization analysis indicated that the cc-viperin protein was located in the cytoplasm. The gene expression results showed that was expressed in all of the tissues tested. Its expression level significantly increased in EPC cells after 24 h to 72 h compared to the control during SVCV infection. Moreover, significantly inhibited SVCV replication when it was overexpressed, whereas it increased SVCV replication when it had reduced expression in EPC cells, respectively. To summarize, the results obtained in this work show that shares similar sequence characteristics with other vertebrates, and it could inhibit SVCV replication in EPC cells, displaying an antiviral effect in common carp.
是一种干扰素刺激基因（ISG），在脊椎动物的先天性抗病毒免疫中发挥重要作用。在本研究中，对鲤鱼（）基因进行了表征，并确定其是否具有抑制鲤鱼春季病毒血症病毒（SVCV）在EPC细胞中复制的能力。结果表明，该基因的全长cDNA为1044 bp，编码348个氨基酸。cc - viperin序列在N端包含一个亮氨酸拉链，在SAM结构域中有一个CxxxCxxC基序，以及一个保守的C端。该基因的核苷酸和氨基酸序列比对显示，与其他物种相比，具有相对较高的序列同一性。系统发育分析显示cc - viperin与和关系密切。亚细胞定位分析表明，cc - viperin蛋白位于细胞质中。基因表达结果表明，在所检测的所有组织中均有表达。在SVCV感染期间，与对照相比，其在EPC细胞中的表达水平在24小时至72小时后显著增加。此外，过表达时显著抑制SVCV复制，而在EPC细胞中表达降低时则增加SVCV复制。综上所述，本研究结果表明，与其他脊椎动物具有相似的序列特征，并且可以抑制EPC细胞中的SVCV复制，在鲤鱼中发挥抗病毒作用。
