Oliveira Sandra C, Araújo Samuel M, Dencheva Nadya V, Denchev Zlatan Z
IPC-Institute for Polymers and Composites, University of Minho, 4800-056 Guimarães, Portugal.
Molecules. 2024 Dec 30;30(1):114. doi: 10.3390/molecules30010114.
Free pectinase is commonly employed as a biocatalyst in wine clarification; however, its removal, recovery, and reuse are not feasible. To address these limitations, this study focuses on the immobilization of a commercial pectinolytic preparation (Pec) onto highly porous polymer microparticles (MPs). Seven microparticulate polyamide (PA) supports, namely PA4, PA6, PA12 (with and without magnetic properties), and the copolymeric PA612 MP, were synthesized through activated anionic ring-opening polymerization of various lactams. Pectinase was non-covalently immobilized on these supports by adsorption, forming Pec@PA conjugates. Comparative activity and kinetic studies revealed that the Pec@PA12 conjugate exhibited more than twice the catalytic efficiency of the free enzyme, followed by Pec@PA6-Fe and Pec@PA4-Fe. All Pec@PA complexes were tested in the clarification of industrial rosé must, demonstrating similar or better performance compared to the free enzyme. Some immobilized biocatalysts supported up to seven consecutive reuse cycles, maintaining up to 50% of their initial activity and achieving complete clarification within 3-30 h across three consecutive cycles of application. These findings highlight the potential for industrial applications of noncovalently immobilized pectinase on various polyamide microparticles, with possibilities for customization of the conjugates' properties.
游离果胶酶通常用作葡萄酒澄清的生物催化剂；然而，其去除、回收和再利用并不可行。为了解决这些限制，本研究重点关注将一种商业果胶分解制剂（Pec）固定在高度多孔的聚合物微粒（MPs）上。通过各种内酰胺的活性阴离子开环聚合反应合成了七种微粒状聚酰胺（PA）载体，即PA4、PA6、PA12（有磁性和无磁性）以及共聚PA612 MP。果胶酶通过吸附非共价固定在这些载体上，形成Pec@PA缀合物。比较活性和动力学研究表明，Pec@PA12缀合物的催化效率是游离酶的两倍多，其次是Pec@PA6-Fe和Pec@PA4-Fe。所有Pec@PA复合物都在工业桃红葡萄汁的澄清中进行了测试，与游离酶相比表现出相似或更好的性能。一些固定化生物催化剂支持连续多达七个重复使用周期，在连续三个应用周期内保持高达其初始活性的50%，并在3 - 30小时内实现完全澄清。这些发现突出了非共价固定在各种聚酰胺微粒上的果胶酶在工业应用中的潜力，以及定制缀合物性质的可能性。
