Munnerlyn C R, Gray J R, Hennings D R
Graefes Arch Clin Exp Ophthalmol. 1985;222(4-5):209-11. doi: 10.1007/BF02133676.
Fluorometric studies have been made with modified slit-lamp microscopes. These instruments offered considerable versatility in modifying experimental parameters, but were seldom optimized in terms of detector and illuminator apertures, filter band-pass characteristics, detection system, light-source stabilization, or optical resolution. Improper control of these parameters can result in artifacts which override useful information. A new fluorophotometer for ocular research has been designed that utilizes an optical system, which eliminates the need for a contact lens. An effort was made to optimize both the axial resolution and the minimum detectable signal. The characteristics of standard barrier filters and excitation filters were examined and compared with photomultiplier sensitivity curves. It was necessary to use double filters in order to reduce artifacts due to excitation light that was not rejected by the barrier filter. In order to optimize the signal-to-noise ratio, a photon counting detection system was used. A dark current equivalent to 3 X 10(-10) g/ml allows a useful detection range of 1 X 10(-10) g/ml.
已使用改良的裂隙灯显微镜进行荧光研究。这些仪器在修改实验参数方面具有相当大的通用性，但在探测器和照明器孔径、滤光片带通特性、检测系统、光源稳定性或光学分辨率方面很少进行优化。对这些参数控制不当会导致伪像，从而掩盖有用信息。已设计出一种用于眼部研究的新型荧光光度计，它采用了一种光学系统，无需使用接触镜。努力优化轴向分辨率和最小可检测信号。研究了标准阻挡滤光片和激发滤光片的特性，并与光电倍增管灵敏度曲线进行了比较。为了减少由于阻挡滤光片未阻挡的激发光引起的伪像，有必要使用双滤光片。为了优化信噪比，使用了光子计数检测系统。相当于3×10（-10）克/毫升的暗电流允许1×10（-10）克/毫升的有用检测范围。
