School of Food Science and Technology, Dalian Polytechnic University, National Engineering Research Center of Seafood, Dalian 116034, China.
Department of Dairy and Food Science, South Dakota State University, Brookings, SD 57007, USA.
Mar Drugs. 2019 Jul 24;17(8):434. doi: 10.3390/md17080434.
sporophyll () is a major byproduct of (a brown algae) processing. Its phenolic constituents, phlorotannins, are of special interest due to their intrinsic ability to precipitate proteins. Herein, a high-temperature extraction procedure was used to isolate these biologically active compounds. The heating temperature, heating time, and extraction solvent (ethanol) concentration were examined with response surface analysis to determine the optimal conditions to achieve the maximum extraction rate. The analysis revealed the optimal conditions to be: 170 °C of heating temperature, 5.2 h of heating time, and 52% ethanol concentration for a yield of 10.7 ± 0.2 mg gallic acid equivalent/g dry weight (GAE/g DW) of sample. Compared to epigallocatechin gallate (EGCG), the extracted phlorotannins displayed higher antioxidant activity on HO-induced RAW 264.7 cells with improved efficiency. Furthermore, the compounds exhibited strong anti-inflammatory activity. These findings potentially can be utilized to guide development of novel functional foods and food supplements from sea-originated resources such as brown algae.
孢子叶（）是（一种褐藻）加工的主要副产品。其酚类成分，岩藻多酚，由于其内在的沉淀蛋白质的能力而受到特别关注。在此，采用高温提取程序来分离这些具有生物活性的化合物。通过响应面分析检查加热温度、加热时间和提取溶剂（乙醇）浓度，以确定达到最大提取率的最佳条件。分析表明，最佳条件为：加热温度 170°C，加热时间 5.2 小时，乙醇浓度 52%，样品的没食子酸当量/克干重（GAE/g DW）得率为 10.7±0.2mg。与表没食子儿茶素没食子酸酯（EGCG）相比，提取的岩藻多酚在 HO 诱导的 RAW 264.7 细胞中表现出更高的抗氧化活性，效率也有所提高。此外，这些化合物表现出很强的抗炎活性。这些发现可能有助于指导新型功能性食品和食品补充剂的开发，这些食品和食品补充剂来源于褐藻等海洋资源。
