Jewehan Ahmad, Salem Nida, Tóth Zoltán, Salamon Pál, Szabó Zoltán
Applied Plant Genomics Group, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Gödöllő, 2100 Hungary.
Department of Plant Protection, School of Agriculture, The University of Jordan, Amman, 11942 Jordan.
J Plant Dis Prot (2006). 2022;129(1):117-123. doi: 10.1007/s41348-021-00535-x. Epub 2021 Oct 9.
The reaction of 636 (sections and ) accessions were evaluated under greenhouse conditions after mechanical inoculation with a Jordanian isolate of the new tobamovirus tomato brown rugose fruit virus (ToBRFV). Local and systemic infections were assayed by symptoms evaluation and virus detection via biotests and RT-PCR. All cultivated tomatoes () and the great majority of wild tomato accessions proved susceptible to ToBRFV. They showed a wide range of symptoms (mosaic, leaf deformations, mottling, shoestring, and stunting). Twenty-six accessions representing var. cerasiforme, , and were tolerant. High levels of resistance have been demonstrated in three accessions of , a close relative to wild tomatoes (member of the sect. ) not only to ToBRFV but also to the tobamoviruses, tobacco mosaic virus (TMV) and tomato mosaic virus (ToMV). After mechanical inoculation, the three tobamoviruses could be detected only in inoculated leaves in the accessions LA2160, LA2162, and LA 2166, which remained symptomless. However, two other accessions PI 473,498 and PI 230,519 reacted unusually. They were demonstrated highly resistant to TMV and ToMV, but proved transiently susceptible to ToBRFV showing mild systemic mosaic followed by total recovery from symptoms and the virus.
The online version contains supplementary material available at 10.1007/s41348-021-00535-x.
在用约旦分离的新型烟草花叶病毒番茄褐色皱纹果病毒（ToBRFV）对636份（品系和品种）材料进行机械接种后，在温室条件下对其反应进行了评估。通过症状评估以及生物检测和逆转录聚合酶链反应（RT-PCR）进行病毒检测，以测定局部和系统感染情况。所有栽培番茄（品种）以及绝大多数野生番茄材料均被证明对ToBRFV敏感。它们表现出广泛的症状（花叶、叶片变形、斑驳、鞋带状和发育迟缓）。代表醋栗番茄变种、多毛番茄、智利番茄和小花番茄的26份材料具有耐受性。已在野生番茄的近缘种（茄属sect.的成员）的三个材料中证明了对ToBRFV以及烟草花叶病毒（TMV）和番茄花叶病毒（ToMV）的高抗性。机械接种后，仅在LA2160、LA2162和LA 2166材料的接种叶片中检测到这三种烟草花叶病毒，这些材料无症状。然而，另外两个番茄材料PI 473,498和PI 230,519反应异常。它们对TMV和ToMV表现出高度抗性，但对ToBRFV表现出暂时易感性，出现轻度系统花叶症状，随后症状和病毒完全恢复。
在线版本包含可在10.1007/s41348-021-00535-x获取的补充材料。
