使用卡那霉素6'-乙酰转移酶对氨基糖苷类进行放射酶测定。
Radioenzymatic assays for aminoglycosides with kanamycin 6'-acetyltransferase.
作者信息
Weber A, Smith A L, Opheim K E
出版信息
J Clin Microbiol. 1985 Mar;21(3):419-24. doi: 10.1128/jcm.21.3.419-424.1985.
To facilitate the rapid and accurate quantitation of parenterally administered aminoglycosides, we defined the optimum conditions (pH, duration of incubation, and cofactor concentrations) to permit radioenzymatic assays with kanamycin acetyltransferase. The accuracy in quantitating tobramycin, netilmicin, kanamycin, and amikacin at concentrations in the therapeutic range was greater than 90%, with a mean recovery of 102.8%. The mean of the interassay coefficient of variation was 7.8%. Typical standard curves at six different concentrations resulted in a correlation coefficient (r value) of greater than 0.99 for each aminoglycoside. The radioenzymatic assay correlates well with the bioassay (tobramycin and netilmicin) and radioimmunoassay (amikacin and kanamycin); the correlation coefficient is greater than 0.90 for all. We conclude that the radioenzymatic assay utilizing kanamycin 6'-acetyltransferase is feasible for all commercially available parenterally administered aminoglycosides.
为便于快速、准确地定量测定胃肠外给药的氨基糖苷类药物，我们确定了最佳条件（pH值、孵育时间和辅因子浓度），以进行卡那霉素乙酰转移酶的放射酶法测定。在治疗范围内的浓度下，对妥布霉素、奈替米星、卡那霉素和阿米卡星进行定量的准确度大于90%，平均回收率为102.8%。批间变异系数的平均值为7.8%。六种不同浓度下的典型标准曲线显示，每种氨基糖苷类药物的相关系数（r值）均大于0.99。放射酶法与生物测定法（妥布霉素和奈替米星）及放射免疫测定法（阿米卡星和卡那霉素）相关性良好；所有方法的相关系数均大于0.90。我们得出结论，利用卡那霉素6'-乙酰转移酶的放射酶法适用于所有市售的胃肠外给药氨基糖苷类药物。
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