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转录组学和代谢组学联合分析揭示头花蓼不同部位黄酮类化合物代谢的生药学机制。

Combined transcriptomic and metabolomic analyses reveal the pharmacognostic mechanism of the metabolism of flavonoids in different parts of Polygonum capitatum.

作者信息

Yang Jie, Zhang Yu, Guo Bu-Fa, Peng Qi-Lun, Chen Hong-Yu, Ye Mao, Yi Wei, Ding Wei-Jun

机构信息

Department of Fundamental Medicine, Bijie Medical College, Bijie, Guizhou, China.

The Key Laboratory for Health Industry, Bijie Medical College, Bijie, Guizhou, China.

出版信息

Plant Genome. 2025 Mar;18(1):e20543. doi: 10.1002/tpg2.20543.

DOI:10.1002/tpg2.20543
PMID:39807534
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11729211/
Abstract

The plant Polygonum capitatum (P. capitatum) contains a variety of flavonoids that are distributed differently among different parts. Nevertheless, differentially expressed genes (DEGs) associated with this heterogeneous distribution have not been identified. In this study, combined with transcriptomic and metabonomic analysis, we identified significant DEGs related to variations in flavonoid composition among different parts of P. capitatum. Subsequently, transcriptomic and nontargeted metabolomic analyses revealed that flavonoids and phenolic acids in different parts of P. capitatum were significantly enriched in the phenylpropanoid biosynthesis, shikimic acid biosynthesis, and flavonoid biosynthesis pathways. The expression levels of genes encoding enzymes, including shikimate O-hydroxycinnamoyltransferase (HCT), chalcone synthase (CHS), flavonoid 3',5'-hydroxylase (CYP75A), flavones 3-hydroxylase (F3H), flavonol synthase (FLS), leucoanthocyanidin reductase (LAR), trans-cinnamate 4-monooxygenase (CYP73A), and shikimate kinase (SK), were found to be the lowest in the leaves of P. capitatum via quantitative PCR. Interestingly, these genes are involved in the biosynthesis of quality markers such as gallic acid, quercetin, and quercitrin in P. capitatum. Finally, the targeted metabolomic results reconfirmed that the gallic acid, quercetin, and quercitrin contents were the highest in the leaves of P. capitatum. This research provides a theoretical basis for further understanding the differential regulatory mechanism of flavonoid metabolism in different parts of P. capitatum, providing novel insights into the pharmacognostic basis of P. capitatum.

摘要

头花蓼含有多种黄酮类化合物，这些黄酮类化合物在不同部位的分布存在差异。然而，与这种异质性分布相关的差异表达基因（DEGs）尚未被鉴定出来。在本研究中，结合转录组学和代谢组学分析，我们鉴定出了与头花蓼不同部位黄酮类化合物组成变化相关的重要DEGs。随后，转录组学和非靶向代谢组学分析表明，头花蓼不同部位的黄酮类化合物和酚酸在苯丙烷生物合成、莽草酸生物合成和黄酮类生物合成途径中显著富集。通过定量PCR发现，编码莽草酸O - 羟基肉桂酰转移酶（HCT）、查尔酮合酶（CHS）、黄酮类3',5'-羟化酶（CYP75A）、黄酮3-羟化酶（F3H）、黄酮醇合酶（FLS）、无色花青素还原酶（LAR）、反式肉桂酸4-单加氧酶（CYP73A）和莽草酸激酶（SK）等酶的基因在头花蓼叶片中的表达水平最低。有趣的是，这些基因参与了头花蓼中没食子酸、槲皮素和槲皮苷等质量标志物的生物合成。最后，靶向代谢组学结果再次证实，头花蓼叶片中没食子酸、槲皮素和槲皮苷的含量最高。本研究为进一步了解头花蓼不同部位黄酮类代谢的差异调控机制提供了理论依据，为头花蓼的生药学基础提供了新的见解。

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