Perović Tatjana, Lazović Biljana, Adakalić Mirjana, Džamić Ana, Žarković Lazar, Gašić Uroš, Kostić Marina, Petrović Jovana, Stojković Dejan, Ćirić Ana
Biotechnical Faculty, Centre for Subtropical Cultures, University of Montenegro, Topolica bb, 8500 Bar, Montenegro.
University of Belgrade, Faculty of Biology, Studentski trg 16, 11000, Belgrade, Serbia.
Heliyon. 2024 Dec 19;11(1):e41361. doi: 10.1016/j.heliyon.2024.e41361. eCollection 2025 Jan 15.
Jujube ( Mill.) is a highly abundant wild-growing plant in Montenegro. It has been utilized since old times for various bioactive properties by the natives, however its detailed chemical characterization, antimicrobial, antioxidant and cytotoxic potential have not been extensively explored. Herein, we used crud methanol extract and three fractions (methylene chloride, -butanol and aqueous) to asses bioactive features of fruits from this wild growing edible plant, after which we performed the targeted analysis of phenolic compounds of -butanol fraction by (UHPLC-DAD-MS/MS). Our antioxidant assays showed the highest radical scavenging potential for -butanol fraction using DPPH and ABTS methods. As for the antimicrobial activity of extract and three fractions, generally aqueous showed the least promising antibacterial and antifungal properties, whereas methylene chloride, methanol and -butanol fractions showed quite promising antimicrobial potential. and strains were the most susceptible to the compounds present in the methylene chloride and -butanol fractions with MIC of 0.01-0.025 mg/mL, and MBC 0.025-0.05 mg/mL, along with MRSA strain, which was the most susceptible to the effects of methanol extract with MIC of 0.10 mg/mL and MBC 0.15 mg/mL. The results of antifungal activity showed lower potential to inhibit growth of pathogenic fungi than bacteria, whereas cytotoxicity assay showed extracts have no effects towards HaCaT skin cell line.The -butanol fraction had the overall most promising activity, and therefore was subjected to more in depth chemical analysis.
枣（Mill.）是黑山一种极为常见的野生植物。当地人自古以来就因其多种生物活性特性而加以利用，然而其详细的化学特征、抗菌、抗氧化和细胞毒性潜力尚未得到广泛研究。在此，我们使用粗甲醇提取物和三个馏分（二氯甲烷、正丁醇和水相）来评估这种野生可食用植物果实的生物活性特征，之后我们通过（超高效液相色谱 - 二极管阵列 - 串联质谱法）对正丁醇馏分中的酚类化合物进行了靶向分析。我们的抗氧化试验表明，使用二苯基苦味酰基自由基（DPPH）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）方法时，正丁醇馏分具有最高的自由基清除潜力。至于提取物和三个馏分的抗菌活性，一般来说水相显示出最不乐观的抗菌和抗真菌特性，而二氯甲烷、甲醇和正丁醇馏分显示出相当可观的抗菌潜力。 和 菌株对二氯甲烷和正丁醇馏分中存在的化合物最为敏感，最低抑菌浓度（MIC）为0.01 - 0.025毫克/毫升，最低杀菌浓度（MBC）为0.025 - 0.05毫克/毫升，耐甲氧西林金黄色葡萄球菌（MRSA）菌株也对甲醇提取物的作用最为敏感，MIC为0.10毫克/毫升，MBC为0.15毫克/毫升。抗真菌活性结果表明，其抑制致病真菌生长的潜力低于细菌，而细胞毒性试验表明提取物对永生化角质形成细胞系（HaCaT）没有影响。正丁醇馏分总体上具有最可观的活性，因此对其进行了更深入的化学分析。
