Zhong Yijia, Zhou Zhie, Yin Zhongping, Zhang Lu, Zhang Qingfeng, Xie Yihai, Chen Jiguang
Jiangxi Key Laboratory of Natural Products and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang, 330045, China.
Collaborative Innovation Center of Jiangxi Typical Trees Cultivation and Utilization, College of Forestry, Jiangxi Agricultural University, Nanchang, 330045, China.
J Plant Physiol. 2025 Feb;305:154431. doi: 10.1016/j.jplph.2025.154431. Epub 2025 Jan 10.
Physalis peruviana L. (P. peruviana) is an edible medicinal plant rich in bioactive phenolics. This study aimed to establish a hairy root (HR) culture of P. peruviana as a potential source for the synthesis of natural compounds. HRs were induced in P. peruviana using different Agrobacterium rhizogenes strains (R1601, C58C1, A4, and K599). Notably, K599 did not induce HR formation, whereas R1601, C58C1, and A4 yielded transformation frequencies of 57.78, 65.14, and 72.31%, respectively. Secondary metabolite production and antioxidant capacity were further examined in HRs induced using C58C1, R1601, and A4. It was found that A. rhizogenes R1601 induced the greatest increase (44% compared to that observed in the non-transformed culture). The methanolic extract of HRs induced by A. rhizogenes R1601 exhibited strong antioxidant capacity, with IC values of 1.41 mg DE/mL and 2.33 mg DE/mL for 2,2-diphenyl-1-picrylhydrazyl and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), respectively. The HR culture showed higher production of phenolic compounds and higher antioxidant capacity than the non-transformed cultures. Ultra-performance liquid chromatography time-of-flight tandem mass spectrometry was used to identify eight alkaloids, phenolics, and glycoside compounds. A. rhizogenes R1601 is emerging as a possible strain for the mass production of HR and bioactive phenolic compounds in P. peruviana.
灯笼果（Physalis peruviana L.）是一种富含生物活性酚类物质的可食用药用植物。本研究旨在建立灯笼果的毛状根（HR）培养体系，作为合成天然化合物的潜在来源。使用不同的发根农杆菌菌株（R1601、C58C1、A4和K599）诱导灯笼果产生毛状根。值得注意的是，K599未诱导出毛状根形成，而R1601、C58C1和A4的转化频率分别为57.78%、65.14%和72.31%。进一步检测了用C58C1、R1601和A4诱导的毛状根中的次生代谢产物产量和抗氧化能力。发现发根农杆菌R1601诱导的增加最为显著（与未转化培养物相比增加了44%）。发根农杆菌R1601诱导的毛状根甲醇提取物表现出较强的抗氧化能力，对2,2-二苯基-1-苦基肼和2,2-联氮双（3-乙基苯并噻唑啉-6-磺酸）的IC值分别为1.41 mg DE/mL和2.33 mg DE/mL。与未转化培养物相比，毛状根培养物显示出更高的酚类化合物产量和更高的抗氧化能力。采用超高效液相色谱飞行时间串联质谱法鉴定了8种生物碱、酚类和糖苷类化合物。发根农杆菌R1601正成为大规模生产灯笼果毛状根和生物活性酚类化合物的一种可能菌株。
