Alsteens David
Louvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Croix du sud 4-5, L7.07.07, 1348 Louvain-la-Neuve, Belgium.
WELBIO Department, WEL Research Institute, Avenue Pasteur, 6, 1300 Wavre, Belgium.
Biophys Rev. 2024 Dec 28;16(6):663-677. doi: 10.1007/s12551-024-01258-7. eCollection 2024 Dec.
Atomic force microscopy (AFM) has emerged as a powerful tool for studying biological interactions at the single-molecule level, offering unparalleled insights into receptor-ligand dynamics on living cells. This review discusses key developments in the application of AFM, highlighting its ability to capture nanomechanical properties of cellular surfaces and probe dynamic interactions, such as virus-host binding. AFM's versatility in measuring mechanical forces and mapping molecular interactions in near-physiological conditions is explored. The review also emphasizes how AFM provides critical insights into cell surface organization, receptor functionality, and viral entry mechanisms, advancing the understanding of cellular and molecular processes.
原子力显微镜（AFM）已成为在单分子水平研究生物相互作用的强大工具，为深入了解活细胞上受体 - 配体动力学提供了无与伦比的视角。本综述讨论了AFM应用中的关键进展，突出了其捕捉细胞表面纳米力学特性以及探测动态相互作用（如病毒 - 宿主结合）的能力。探讨了AFM在近生理条件下测量机械力和绘制分子相互作用图谱方面的多功能性。该综述还强调了AFM如何为细胞表面组织、受体功能和病毒进入机制提供关键见解，推动了对细胞和分子过程的理解。
