Zhang Miao, Zhang Ming, Han Ruixue, Yu Xin, Song Zhaolu
Department of Respiratory, Jiaozhou Central Hospital of Qingdao, Qingdao, 266300, China.
Department of Clinical Medicine, Anhui Medical College, Hefei, 230601, China.
J Cardiothorac Surg. 2025 Jan 20;20(1):76. doi: 10.1186/s13019-024-03322-5.
There are still gaps in the study of the miRNA and its SNPs in some diseases such as non-small cell lung cancer (NSCLC). The study aimed to provide useful information on the treatment of NSCLC by investigating the association between miR-21 and its SNPs and NSCLC susceptibility.
The serum of NSCLC patients (n = 205) and cancer-free controls (n = 217) were collected in this study for RNA extraction. The qRT-PCR was used to evaluate the expression of miR-21 and Taqman qPCR was used for genotyping and quantifying miR-21 SNPs (rs1292037, rs6504593). The association of the expression of miR-21, the miR-21 SNPs and their interactions with the susceptibility of NSCLC patients were analysed using logistic regression analysis in this study.
This study showed that the overexpression of miR-21 was related to NSCLC. The C allele and CC genotypes of rs1292037 and rs6504593 were associated with the increased risk of NSCLC susceptibility. Moreover, the interactions of rs1292037 and rs6504593 were also a risk factor for NSCLC. The CC genotypes of rs1292037 and rs6504593 were associated with the increase of miR-21 expression.
The overexpression of miR-21, the miR-21 SNPs rs1292037 and rs6504593 and their interactions were associated with NSCLC susceptibility. MiR-21 and its SNPs have potential for being targets in the therapy of NSCLC. This study provided important information for the treatment of NSCLC.
在某些疾病如非小细胞肺癌（NSCLC）中，关于微小RNA（miRNA）及其单核苷酸多态性（SNP）的研究仍存在空白。本研究旨在通过调查miR-21及其SNP与NSCLC易感性之间的关联，为NSCLC的治疗提供有用信息。
本研究收集了205例NSCLC患者和217例无癌对照者的血清用于RNA提取。采用qRT-PCR评估miR-21的表达，Taqman qPCR用于对miR-21 SNP（rs1292037、rs6504593）进行基因分型和定量。本研究使用逻辑回归分析来分析miR-21的表达、miR-21 SNP及其相互作用与NSCLC患者易感性之间的关联。
本研究表明miR-21的过表达与NSCLC相关。rs1292037和rs6504593的C等位基因和CC基因型与NSCLC易感性风险增加相关。此外，rs1292037和rs6504593的相互作用也是NSCLC的一个危险因素。rs1292037和rs6504593的CC基因型与miR-21表达增加相关。
miR-21过表达、miR-21 SNP rs1292037和rs6504593及其相互作用与NSCLC易感性相关。MiR-21及其SNP有潜力成为NSCLC治疗的靶点。本研究为NSCLC的治疗提供了重要信息。
