Oyster Mushroom () Ethanolic Extract Inhibits Pparg Expression While Maintaining the Methylation of the Promoter During 3T3-L1 Adipocyte Differentiation.

PURPOSE

This study aims to provide new insights into the potential of oyster mushroom () ethanolic extract in preventing obesity through the inhibition of expression and modulation of methylation level on promoter during 3T3-L1 adipocyte differentiation.

METHODS

This in vitro quantitative experimental study was conducted by treating the 3T3-L1 cell line differentiated using 0.5 mM methyl-isobutyl-xanthine, 1 μM dexamethasone, and 10 μg/mL insulin-containing medium with oyster mushroom ethanolic extract. The extract was obtained from 80 g of dried oyster mushroom powder extracted three times with 800 mL of ethanol, filtered, evaporated, and reconstituted in dimethyl sulfoxide (DMSO) to final concentrations of 0, 25, 50, and 100 µg/mL, with DMSO limited to 0.5% in all solutions. mRNA expression was quantified by qRT-PCR analysis and promoter methylation levels were measured quantitatively by pyrosequencing of bisulfite-treated DNA samples.

RESULTS

The addition of 25 µg/mL oyster mushroom ethanolic extract significantly suppressed mRNA expression with no significant change in the promoter methylation levels.

CONCLUSION

Oyster mushroom ethanolic extract inhibited mRNA expression without altering promoter methylation, suggesting reduced adipocyte differentiation. This study emphasizes the potential of oyster mushroom in the prevention or treatment of obesity by inhibiting adipocyte differentiation.