Ariyanto Eko Fuji, Farahana Anastasya Kania, Sudirman Gabriella Sachiko Jannesha, Widiarsih Erlina, Qomarilla Nurul, Rahayu Nurul Setia, Wikayani Tenny Putri, Heryaman Henhen, Wira Dwi Wahyudha, Triatin Rima Destya, Bashari Muhammad Hasan, Pamela Yunisa, Pratiwi Yuni Susanti, Ghozali Mohammad
Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Sumedang, West Java, Indonesia.
Research Center for Medical Genetics, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia.
J Exp Pharmacol. 2025 Jan 16;17:27-36. doi: 10.2147/JEP.S494116. eCollection 2025.
This study aims to provide new insights into the potential of oyster mushroom () ethanolic extract in preventing obesity through the inhibition of expression and modulation of methylation level on promoter during 3T3-L1 adipocyte differentiation.
This in vitro quantitative experimental study was conducted by treating the 3T3-L1 cell line differentiated using 0.5 mM methyl-isobutyl-xanthine, 1 μM dexamethasone, and 10 μg/mL insulin-containing medium with oyster mushroom ethanolic extract. The extract was obtained from 80 g of dried oyster mushroom powder extracted three times with 800 mL of ethanol, filtered, evaporated, and reconstituted in dimethyl sulfoxide (DMSO) to final concentrations of 0, 25, 50, and 100 µg/mL, with DMSO limited to 0.5% in all solutions. mRNA expression was quantified by qRT-PCR analysis and promoter methylation levels were measured quantitatively by pyrosequencing of bisulfite-treated DNA samples.
The addition of 25 µg/mL oyster mushroom ethanolic extract significantly suppressed mRNA expression with no significant change in the promoter methylation levels.
Oyster mushroom ethanolic extract inhibited mRNA expression without altering promoter methylation, suggesting reduced adipocyte differentiation. This study emphasizes the potential of oyster mushroom in the prevention or treatment of obesity by inhibiting adipocyte differentiation.
本研究旨在通过抑制3T3-L1脂肪细胞分化过程中 的表达以及调节 启动子的甲基化水平，为平菇乙醇提取物在预防肥胖方面的潜力提供新的见解。
本体外定量实验研究通过用平菇乙醇提取物处理使用0.5 mM甲基异丁基黄嘌呤、1 μM地塞米松和含10 μg/mL胰岛素的培养基分化的3T3-L1细胞系来进行。该提取物从80 g干平菇粉中获得，用800 mL乙醇提取三次，过滤、蒸发，然后在二甲基亚砜（DMSO）中重构至最终浓度为0、25、50和100 μg/mL，所有溶液中DMSO含量限制在0.5%。通过qRT-PCR分析定量 mRNA表达，并通过对亚硫酸氢盐处理的DNA样本进行焦磷酸测序定量 启动子甲基化水平。
添加25 μg/mL平菇乙醇提取物显著抑制 mRNA表达，而 启动子甲基化水平无显著变化。
平菇乙醇提取物抑制 mRNA表达而不改变 启动子甲基化，提示脂肪细胞分化减少。本研究强调了平菇在通过抑制脂肪细胞分化预防或治疗肥胖方面的潜力。
