Guo Wen, Zhang Xinyue, Wan Long, Wang Zhiqi, Han Meiqi, Yan Ziwei, Li Jia, Deng Ruizhu, Li Shenglong, Mao Yuling, Wang Siling
Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, China.
Department of Pharmacy, The First Hospital of China Medical University, 155 Nanjing North Street, Shenyang 110001, Liaoning, China.
J Pharm Anal. 2024 Dec;14(12):100953. doi: 10.1016/j.jpha.2024.02.007. Epub 2024 Feb 23.
Particle size and surface properties are crucial for lymphatic drainage (LN), dendritic cell (DC) uptake, DC maturation, and antigen cross-presentation induced by nanovaccine injection, which lead to an effective cell-mediated immune response. However, the manner in which the particle size and surface properties of vaccine carriers such as mesoporous silica nanoparticles (MSNs) affect this immune response is unknown. We prepared 50, 100, and 200 nm of MSNs that adsorbed ovalbumin antigen (OVA) while modifying -glucan to enhance immunogenicity. The results revealed that these MSNs with different particle sizes were just as efficient , and MSNs with -glucan modification demonstrated higher efficacy. However, the results indicated that MSNs with smaller particle sizes have stronger lymphatic targeting efficiency and a greater ability to promote the maturation of DCs. The results also indicate that -glucan-modified MSN, with a particle size of ∼100 nm, has a great potential as a vaccine delivery vehicle and immune adjuvant and offers a novel approach for the delivery of multiple therapeutic agents that target other lymph-mediated diseases.
粒径和表面性质对于纳米疫苗注射诱导的淋巴引流（LN）、树突状细胞（DC）摄取、DC成熟以及抗原交叉呈递至关重要，这些过程会引发有效的细胞介导免疫反应。然而，诸如介孔二氧化硅纳米颗粒（MSN）等疫苗载体的粒径和表面性质影响这种免疫反应的方式尚不清楚。我们制备了吸附卵清蛋白抗原（OVA）的50、100和200纳米的MSN，同时修饰β-葡聚糖以增强免疫原性。结果显示，这些不同粒径的MSN效率相同，而经β-葡聚糖修饰的MSN显示出更高的效率。然而，结果表明粒径较小的MSN具有更强的淋巴靶向效率和促进DC成熟的更大能力。结果还表明，粒径约为100纳米的β-葡聚糖修饰的MSN作为疫苗递送载体和免疫佐剂具有巨大潜力，并为递送针对其他淋巴介导疾病的多种治疗剂提供了一种新方法。
