Zhang Zhiying, Liu Lijun, Wang Huan, Xie Wangni, Zhai Wenhao, Wen Linlin, Zhang Boya, Liu Kexuan, Zhang Xue, Liu Shuchen, Huang Lei, Li Daowei, Zhou Yanmin
Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, 130021, People's Republic of China.
State Key Laboratory of Rare Earth Resources Utilization and Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, People's Republic of China.
Int J Nanomedicine. 2025 May 1;20:5693-5707. doi: 10.2147/IJN.S496921. eCollection 2025.
Large full-thickness skin wounds pose significant challenges, particularly in achieving scar-free healing and the regeneration of skin appendages. This study introduces a portable approach for promoting scarless healing and skin appendage regeneration by utilizing low-intensity pulsed ultrasound (LIPUS) activated piezoelectric electrospun membranes to modulate the local electrical environment.
Dopamine-modified polyvinylidene fluoride (DA/PVDF) nanomembranes were fabricated via electrospinning, followed by piezoelectric characterization under varying LIPUS stimulation. Cell adhesion was examined using SEM and laser confocal microscopy to assess surface interactions. Cell proliferation and migration were further analyzed using the CCK-8 assay and Transwell migration assay, respectively. Finally, the effects of DA/PVDF membranes on full-thickness skin defect healing were tested in a mouse model. The healing process was documented with photographs, and functional skin regeneration was evaluated through histological analysis.
The DA/PVDF nanomembranes had an average diameter of 732 ± 232 nm and generated a voltage of 450 mV under LIPUS stimulation, a 1.28-fold increase compared to PVDF membranes alone. In vitro, LIPUS-activated membranes enhanced cell adhesion and proliferation, resulting in a 1.14-fold increase in cell growth over three days. The transwell migration assays showed 244.67 ± 7.85 migrated cells. , the DA/PVDF+LIPUS group exhibited significantly higher wound healing rates, with improved epidermal regeneration, collagen fiber deposition and remodeling, and enhanced blood vessel and skin appendage formation.
DA modification enhances the piezoelectric properties of PVDF membranes, boosting cell adhesion and promoting dermal and vascular regeneration. LIPUS-generated mechanical waves effectively stimulate membrane deformation, producing a localized electrical microenvironment that mimics the natural bioelectric field of skin and accelerates functional wound healing.
大面积全层皮肤伤口带来了重大挑战，尤其是在实现无瘢痕愈合和皮肤附属器再生方面。本研究介绍了一种便携式方法，通过利用低强度脉冲超声（LIPUS）激活的压电静电纺丝膜来调节局部电环境，以促进无瘢痕愈合和皮肤附属器再生。
通过静电纺丝制备多巴胺修饰的聚偏氟乙烯（DA/PVDF）纳米膜，随后在不同的LIPUS刺激下进行压电特性表征。使用扫描电子显微镜（SEM）和激光共聚焦显微镜检查细胞黏附情况，以评估表面相互作用。分别使用CCK-8法和Transwell迁移试验进一步分析细胞增殖和迁移情况。最后，在小鼠模型中测试DA/PVDF膜对全层皮肤缺损愈合的影响。用照片记录愈合过程，并通过组织学分析评估功能性皮肤再生情况。
DA/PVDF纳米膜的平均直径为732±232nm，在LIPUS刺激下产生450mV的电压，比单独的PVDF膜增加了1.28倍。在体外，LIPUS激活的膜增强了细胞黏附和增殖，在三天内细胞生长增加了1.14倍。Transwell迁移试验显示有244.67±7.85个迁移细胞。DA/PVDF+LIPUS组的伤口愈合率显著更高，表皮再生、胶原纤维沉积和重塑得到改善，血管和皮肤附属器形成增强。
DA修饰增强了PVDF膜的压电性能，促进细胞黏附并促进真皮和血管再生。LIPUS产生的机械波有效地刺激膜变形，产生局部电微环境，模拟皮肤的自然生物电场并加速功能性伤口愈合。
