Shchennikova A V, Kochieva E Z, Filyushin M A
Institute of Bioengineering, Federal Research Center "Fundamentals of Biotechnology" of the Russian Academy of Sciences, Moscow, Russia.
Dokl Biochem Biophys. 2025 Feb;520(1):49-52. doi: 10.1134/S1607672924601057. Epub 2025 Jan 22.
The expression profile of the key genes of biosynthesis (VTC2, GPP, GalDH, and GalLDH) and recycling (MDHAR1, MDHAR4, and MDHAR5) of ascorbate in response to infection with the fungal pathogen Fusarium proliferatum in garlic cultivars resistant (Podnebesny) and sensitive (Dubkovsky) to Fusarium rot was determined. It was found that differences in resistance to Fusarium lead to discrepancies in the dynamics and expression of individual genes of the ascorbate pathway, as well as in the ascorbate content. It was shown that, in response to infection, the expression level of the MDHAR4 gene increases in the resistant cultivar and decreases in the Fusarium-sensitive accession. As infection progresses, the expression levels of the VTC2 and GalLDH genes increase significantly (higher in the cv. Dubkovsky than in the cv. Podnebesny). In both cultivars, the ascorbate content increases (1.5 times higher in the cv. Dubkovsky than in the cv. Podnebesny).
测定了抗镰刀菌腐烂的大蒜品种（Podnebesny）和敏感品种（Dubkovsky）中，抗坏血酸生物合成关键基因（VTC2、GPP、GalDH和GalLDH）以及循环利用关键基因（MDHAR1、MDHAR4和MDHAR5）在受到真菌病原体层出镰刀菌感染时的表达谱。研究发现，对镰刀菌抗性的差异导致抗坏血酸途径中各个基因的动态变化和表达以及抗坏血酸含量存在差异。结果表明，在感染后，抗性品种中MDHAR4基因的表达水平升高，而对镰刀菌敏感的品种中该基因表达水平降低。随着感染的进展，VTC2和GalLDH基因的表达水平显著增加（Dubkovsky品种比Podnebesny品种更高）。在两个品种中，抗坏血酸含量均增加（Dubkovsky品种比Podnebesny品种高1.5倍）。
