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颅内动脉瘤患者肠道微生物群与代谢组学关联研究方案

Protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms.

作者信息

Xu Hongyu, Jia Zetian, Liu Junhui, Liu Runming, Wei Wei, Li Xiang

机构信息

Brain Research Center, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China; Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China.

Brain Research Center, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China; Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China.

出版信息

STAR Protoc. 2025 Mar 21;6(1):103582. doi: 10.1016/j.xpro.2024.103582. Epub 2025 Jan 22.

DOI:10.1016/j.xpro.2024.103582
PMID:39847486
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11794157/
Abstract

Gut-microbiome-combined metabolomics studies in cerebrovascular disease highlight the microbiota-gut-brain axis in neurological disorders. Here, we present a protocol for correlating the gut microbiome and metabolomics in patients with intracranial aneurysms. We describe steps for sample collection, fecal genomic DNA extraction, rRNA PCR amplification, sequencing library construction, and rRNA sequencing. We then detail procedures for metabolite extraction, liquid chromatography-tandem mass spectrometry (LC-MS/MS) non-targeted metabolomics sequencing, and ELISA for cerebrospinal fluid and plasma samples. Finally, we perform combined multi-omics analysis. For complete details on the use and execution of this protocol, please refer to Xu et al..

摘要

脑血管疾病中肠道微生物群联合代谢组学研究突出了神经疾病中的微生物群-肠道-脑轴。在此，我们提出了一种用于颅内动脉瘤患者肠道微生物群与代谢组学关联分析的方案。我们描述了样本采集、粪便基因组DNA提取、rRNA PCR扩增、测序文库构建和rRNA测序的步骤。然后，我们详细介绍了代谢物提取、液相色谱-串联质谱（LC-MS/MS）非靶向代谢组学测序以及脑脊液和血浆样本酶联免疫吸附测定（ELISA）的程序。最后，我们进行联合多组学分析。有关本方案使用和执行的完整详细信息，请参阅Xu等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/3bff0212d14c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/df4c6ea6970f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/fc64fcd9da3d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/fabcbb9f1ee6/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/93c9f8640651/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/bd71976ee594/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/3bff0212d14c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/df4c6ea6970f/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/fc64fcd9da3d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/fabcbb9f1ee6/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/93c9f8640651/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/bd71976ee594/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c5f/11794157/3bff0212d14c/gr5.jpg

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本文引用的文献

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Gut microbiome-targeted therapies for Alzheimer's disease.针对阿尔茨海默病的肠道微生物组靶向治疗。
Gut Microbes. 2023 Dec;15(2):2271613. doi: 10.1080/19490976.2023.2271613. Epub 2023 Nov 7.
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The role of the gut microbiome and its metabolites in cerebrovascular diseases.肠道微生物群及其代谢产物在脑血管疾病中的作用。
Front Microbiol. 2023 Apr 14;14:1097148. doi: 10.3389/fmicb.2023.1097148. eCollection 2023.
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Gut-on-chip for ecological and causal human gut microbiome research.用于生态和因果性人类肠道微生物组研究的芯片肠道模型
Trends Microbiol. 2022 Aug;30(8):710-721. doi: 10.1016/j.tim.2022.01.014. Epub 2022 Feb 18.
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PICRUSt2 for prediction of metagenome functions.用于宏基因组功能预测的PICRUSt2
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Impact of storage conditions on the human stool metabolome and lipidome: Preserving the most accurate fingerprint.储存条件对人粪便代谢组和脂质组的影响：保存最准确的指纹图谱。
Anal Chim Acta. 2020 Apr 29;1108:79-88. doi: 10.1016/j.aca.2020.02.046. Epub 2020 Feb 26.
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