Jone Md Jahid Hasan, Siddique Md Nure Adil, Biswas Manosh Kumar, Hossain Mohammad Rashed
Plant Molecular Breeding and Bioinformatics Laboratory, Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Department of Genetics and Genome Biology, University of Leicester, Leicester, UK.
Genes Genomics. 2025 Mar;47(3):367-382. doi: 10.1007/s13258-025-01617-y. Epub 2025 Jan 23.
TCP proteins are plant-specific transcription factors that play essential roles in various developmental processes, including leaf morphogenesis and senescence, flowering, lateral branching, hormone crosstalk, and stress responses. However, a comprehensive analysis of genome-wide TCP genes and their expression patterns in melon is yet to be done.
The present study aims to identify and analyze the TCP genes in the melon genome and understand their putative functions.
The chromosomal location, gene structure, conserved motifs, protein domains, structural homology, cis-regulating elements, transcript expression patterns, and potential protein-protein interactions were analyzed using various databases and webtools.
A total of 29 putative TCP genes are identified in melon. These genes were classified into two classes: Class-I (13 genes) and Class-II (16 genes). The results revealed that the putative CmTCP genes are distributed across nine of the twelve melon chromosomes and exhibit diverse expression patterns in different tissues which mostly indicates their potential role in floral organ development, lateral branching, growth and development. Phylogenetic analysis suggests that some CmTCP genes may have similar functions to their homologs in other plant species, while others may have undergone functional diversification.
This study paves the way for future investigations into the specific roles of individual CmTCP genes in melon and for elucidating the mechanisms by which TCP proteins regulate leaf elongation, floral development, and lateral branching.
TCP蛋白是植物特有的转录因子，在包括叶片形态发生与衰老、开花、侧枝形成、激素相互作用以及胁迫反应等多种发育过程中发挥着重要作用。然而，对甜瓜全基因组TCP基因及其表达模式的全面分析尚未开展。
本研究旨在鉴定和分析甜瓜基因组中的TCP基因，并了解其假定功能。
利用各种数据库和网络工具对染色体定位、基因结构、保守基序、蛋白结构域、结构同源性、顺式调控元件、转录本表达模式以及潜在的蛋白-蛋白相互作用进行分析。
在甜瓜中总共鉴定出29个假定的TCP基因。这些基因被分为两类：I类（13个基因）和II类（16个基因）。结果显示，假定的CmTCP基因分布在甜瓜12条染色体中的9条上，且在不同组织中呈现出多样的表达模式，这大多表明它们在花器官发育、侧枝形成、生长和发育中具有潜在作用。系统发育分析表明，一些CmTCP基因可能与其在其他植物物种中的同源基因具有相似功能，而另一些可能经历了功能多样化。
本研究为未来深入探究单个CmTCP基因在甜瓜中的具体作用以及阐明TCP蛋白调控叶片伸长、花发育和侧枝形成的机制铺平了道路。
