Shang Xiaowen, Han Zhaolan, Zhang Dayan, Wang Ya, Qin Hao, Zou Zhongwei, Zhou Lin, Zhu Xujun, Fang Wanping, Ma Yuanchun
College of Horticulture, Nanjing Agricultural University, Nanjing, China.
School of Life Sciences, Southern University of Science and Technology, Shenzhen, China.
Front Plant Sci. 2022 Jul 1;13:840350. doi: 10.3389/fpls.2022.840350. eCollection 2022.
TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors TEOSINTE BRANCHED1/CYCLOIDEA/PCF have been suggested to control the cell growth and proliferation in meristems and lateral organs. A total of 37 genes were identified and divided into two classes, class I (PCF, group 1) and class II (CIN CYC/TB1, groups 2, and 3). The residues of TEOSINTE BRANCHED1/CYCLOIDEA/PCF of Camellia sinensis (Tea plant) (CsTCP) proteins between class I and class II were definitely different in the loop, helix I, and helix II regions; however, eighteen conserved tandem was found in bHLH. There are a large number of homologous gene pairs in three groups. Additionally, most CsTCP proteins have obvious differences in motif composition. The results illuminated that CsTCP proteins in different groups are supposed to have complementary functions, whereas those in the same class seem to display function redundancies. There is no relationship between the number of gene members and genome size, and the gene family has only expanded since the divergence of monocots and eudicots. WGD/segmental duplication played a vital role in the expansion of the gene family in tea plant, and the gene family has expanded a lot. Most genes of group 1 are more widely and non-specifically expressed, and the genes of group 2 are mainly expressed in buds, flowers, and leaves. Most genes of group 1 and some genes of group 2 were up-/downregulated in varying degrees under different stress, genes of group 3 basically do not respond to stress. genes involved in abiotic stress response mostly belong to PCF group. Some genes may have the same function as the homologous genes in Arabidopsis, but there is functional differentiation.
玉米分枝1/轮状花/PCF（TCP）转录因子玉米分枝1/轮状花/PCF被认为可控制分生组织和侧生器官中的细胞生长和增殖。共鉴定出37个基因，并分为两类，即I类（PCF，第1组）和II类（CIN CYC/TB1，第2组和第3组）。茶树（CsTCP）I类和II类蛋白在环、螺旋I和螺旋II区域的玉米分枝1/轮状花/PCF残基明显不同；然而，在bHLH中发现了18个保守串联。三组中有大量同源基因对。此外，大多数CsTCP蛋白在基序组成上有明显差异。结果表明，不同组的CsTCP蛋白应具有互补功能，而同一类中的蛋白似乎表现出功能冗余。基因成员数量与基因组大小之间没有关系，并且该基因家族自单子叶植物和双子叶植物分化以来才开始扩张。全基因组复制/片段重复在茶树基因家族的扩张中起了重要作用，并且该基因家族已经大量扩张。第1组的大多数基因表达更广泛且非特异性，第2组的基因主要在芽、花和叶中表达。第1组的大多数基因和第2组的一些基因在不同胁迫下有不同程度的上调/下调，第3组的基因基本不响应胁迫。参与非生物胁迫响应的基因大多属于PCF组。一些基因可能与拟南芥中的同源基因具有相同功能，但存在功能分化。
