Department of Biological Sciences and Biotechnology, Hannam University Jeonmin-dong, Yuseong-gu, Daejeon 34430, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005226.
A flagella bacterium, designated strain 202IG2-18 was isolated from a marine sponge from Ulleung-do in the Republic of Korea. Cells were Gram-stain-negative, motile, aerobic, rod-shaped and non-pigmented. The strain was able to grow at pH 5.5-9.5 (optimum, pH 7.5), in the presence of 1-5 % (w/v) NaCl (optimum, 3 %, w/v) and at 18-30 °C (optimum, 30 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 202IG2-18 belonged to the family , and was most closely related to [] NRRL B-41466 (97.9 %), followed by KCCM 43304 (97.1 %), KCTC 62318 (96.3 %) and KCTC 22492 (96.2 %). The predominant fatty acids were iso-C (25.7 %), C 8 (13.5 %), summed feature 3 (C 7 and/or C 6, 12.7 %), iso-C (10.4 %) and C (9.6 %). The only detected respiratory quinone was ubiquinone Q-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, three unidentified glycolipids, two unidentified aminophospholipids, an unidentified phospholipid and an unidentified aminolipid. The G+C content of the genomic DNA was 39.8 mol%. The average nucleotide identity values compared to all other related species was below 72.8 % and digital DNA-DNA hybridization values were 21.1-22.3 %, all below the threshold for bacterial species delineation. Phenotypic, phylogenetic, genomic and chemotaxonomic characteristics showed that strain 202IG2-18 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 202IG2-18 (=KACC 22256=LMG 32203).
从韩国郁陵岛的海洋海绵中分离到一株鞭毛菌，命名为 202IG2-18 株。细胞革兰氏阴性，运动，需氧，杆状，非色素。该菌株能够在 pH5.5-9.5（最佳，pH7.5）、1-5%（w/v）NaCl（最佳，3%，w/v）和 18-30°C（最佳，30°C）下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 202IG2-18 属于 科，与 []NRRL B-41466（97.9%）最为密切相关，其次是 []KCCM 43304（97.1%）、[]KCTC 62318（96.3%）和 []KCTC 22492（96.2%）。优势脂肪酸为 iso-C（25.7%）、C 8（13.5%）、summed feature 3（C 7 和/或 C 6，12.7%）、iso-C（10.4%）和 C（9.6%）。唯一检测到的呼吸醌是 ubiquinone Q-8。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油、三种未鉴定的糖脂、两种未鉴定的胺磷脂、一种未鉴定的磷脂和一种未鉴定的胺脂。基因组 DNA 的 G+C 含量为 39.8mol%。与所有其他相关种的平均核苷酸同一性值均低于 72.8%，数字 DNA-DNA 杂交值为 21.1-22.3%，均低于细菌种划分的阈值。表型、系统发育、基因组和化学分类特征表明，菌株 202IG2-18 代表了 属的一个新种，提议命名为 sp. nov.。模式菌株为 202IG2-18（=KACC 22256=LMG 32203）。
