Deng Yi, Dong Zhi Xiang, Yang Gao Hua, Krimsky William S, Tai Yong Hang, Peng Hao, Huang Gui Ting, Xu Jia Xin, Sarkar Saiyad A, Peng Jun, Qian Kai
Faculty of Life and Biotechnology, Kunming University of Science and Technology, Kunming, China.
The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.
Front Immunol. 2025 Jan 9;15:1482084. doi: 10.3389/fimmu.2024.1482084. eCollection 2024.
Dysbiosis of the lung microbiome can contribute to the initiation and progression of lung cancer. Synchronous multiple primary lung cancer (sMPLC) is an increasingly recognized subtype of lung cancer characterized by high morbidity, difficulties in early detection, poor prognosis, and substantial clinical challenges. However, the relationship between sMPLC pathogenesis and changes in the lung microbiome remains unclear.
In this study, 16S rRNA sequencing was performed on clinical samples to analyze lung microbiome composition. Real-time quantitative PCR (qPCR) was used to quantify bacterial abundance in lung tissues. In addition, flow cytometry was conducted to evaluate cell cycle progression and apoptosis in lung tumor cells.
Clinical cohort studies demonstrated that sMPLC occurrence is associated with disturbances in the lung microbiome. Notably, Streptococcus intermedius was enriched in the lungs of sMPLC patients compared with non-tumor controls and accumulated preferentially in tumor tissues. shortened the cell cycle and inhibited apoptosis in lung cancer cells. Analyses of oral and gut microbiomes in different patient cohorts revealed a strong correlation between oral microbiome imbalances and lung microbiome composition in sMPLC patients.
These findings characterize the lung microbiota in sMPLC and identify as a potentially influential bacterial strain. This study provides significant new insights into the diagnosis and treatment of sMPLC.
肺部微生物群失调可促进肺癌的发生和发展。同步性多原发性肺癌（sMPLC）是一种越来越被认可的肺癌亚型，其特点是发病率高、早期检测困难、预后差以及存在重大临床挑战。然而，sMPLC发病机制与肺部微生物群变化之间的关系仍不清楚。
在本研究中，对临床样本进行16S rRNA测序以分析肺部微生物群组成。采用实时定量PCR（qPCR）对肺组织中的细菌丰度进行定量。此外，进行流式细胞术以评估肺肿瘤细胞的细胞周期进程和凋亡情况。
临床队列研究表明，sMPLC的发生与肺部微生物群紊乱有关。值得注意的是，与非肿瘤对照相比，中间链球菌在sMPLC患者的肺部富集，并优先在肿瘤组织中积聚。缩短了肺癌细胞的细胞周期并抑制其凋亡。对不同患者队列的口腔和肠道微生物群分析显示，sMPLC患者口腔微生物群失衡与肺部微生物群组成之间存在强烈相关性。
这些发现描述了sMPLC中的肺部微生物群特征，并确定为一种具有潜在影响力的细菌菌株。本研究为sMPLC的诊断和治疗提供了重要的新见解。
