Mosca Marion, Legain Mélanie, Noël Guillaume, Idée Adrien, Pin Didier
Dermatology Department, Interaction Cell Environment, VetAgro Sup, Université de Lyon, UPSP 2016.A104, 69280 Marcy l'Etoile, France.
Institut Claude Bourgelat ICLB, VetAgro Sup, Université de Lyon, 69280 Marcy l'Etoile, France.
Vet Sci. 2023 Sep 1;10(9):547. doi: 10.3390/vetsci10090547.
To evaluate the skin barrier, the (SC) must be isolated and extracted. Currently, skin biopsy is the gold standard method to investigate skin immunology and the presence of biomarkers in dogs. However, a standardized, non-invasive tool to exclusively remove the SC would be of great interest to study healthy and atopic dogs. In this study, we performed D-squames tape stripping with standardized pressure on seven healthy beagle dogs. A control site was defined and then 25 strips, 50 strips and as many strips as needed to achieve a shiny appearance of the skin were performed on three different experimental sites. After stripping, blinded histopathological examination of a skin biopsy from each site was performed. The number of tape strips required for the skin to become shiny varied between individuals, with a mean of 40 (29-50) strips. There was no significant difference in SC depth between the control site and the site that underwent 25 tape strips. In contrast, the use of 50 strips removed almost all of the SC, with a mean remaining SC depth of 7.82 µm. These data suggest that this non-invasive method can effectively remove the SC, with individual variability, and that a shiny appearance of the skin after stripping can be used as an accurate marker of SC removal.
为评估皮肤屏障，必须分离并提取角质层（SC）。目前，皮肤活检是研究犬类皮肤免疫学和生物标志物存在情况的金标准方法。然而，一种专门用于去除SC的标准化、非侵入性工具对于研究健康犬和特应性犬将非常有意义。在本研究中，我们对七只健康的比格犬施加标准化压力进行D-角质层胶带剥离。定义了一个对照部位，然后在三个不同的实验部位分别进行25次、50次以及为使皮肤呈现光泽所需数量的胶带剥离。剥离后，对每个部位的皮肤活检样本进行盲法组织病理学检查。皮肤变得有光泽所需的胶带剥离次数因个体而异，平均为40（29 - 50）次。对照部位与进行25次胶带剥离的部位之间的SC深度无显著差异。相比之下，使用50次胶带剥离几乎去除了所有的SC，剩余SC的平均深度为7.82 µm。这些数据表明，这种非侵入性方法可以有效去除SC，但存在个体差异，并且剥离后皮肤呈现光泽可作为SC去除的准确标志。
