Takara E, Takeyama E, Jimbo M, Kitamura K
No To Shinkei. 1977 Jul;29(7):804-6.
An attempt was made to collect the cerebrospinal fluid (CSF) of rat using a capillary phenomenon technique. Experiments were carried out in 76 rats anesthetized with pentobarbital sodium (Nembutal, Abbot. 50 mg/kg IP). The midline incision in the occipital region was made from 0.5 cm anterior to interauricular line of 4 cm in length. Exposing the atlanto-occipital dura mater by separating the nuchal muscles, the CSF was collected with a heparinized microtube (MICROPET. Bection, Dickson and Company) through a small hole made by a 18G syringe needle. Thus, 0.05-0.05 ml of the CSF could be readily obtained without mixture of the blood in 64 cases out of 76 trials, 84%. This simple technique is a useful way to get a small amount of CSF from little animals, such as rats.
尝试使用毛细现象技术采集大鼠的脑脊液（CSF）。实验在76只经戊巴比妥钠（Nembutal，雅培公司。50mg/kg腹腔注射）麻醉的大鼠身上进行。在枕部做中线切口，从耳间线前方0.5cm处开始，长4cm。通过分离颈部肌肉暴露寰枕硬脑膜，用肝素化的微量管（MICROPET，贝克顿、迪金森和公司）通过18G注射器针头制成的小孔收集脑脊液。因此，在76次试验中的64例（84%）中，能够轻松获得0.05 - 0.05ml的脑脊液，且无血液混入。这种简单的技术是从小动物如大鼠身上获取少量脑脊液的一种有用方法。
