Falkauskas Rimvydas, Jovaišienė Jurgita, Vaičiulienė Gintarė, Kerzienė Sigita, Jacevičienė Ingrida, Jacevičius Eugenijus, Jarmalaitė Inga, Ivaškienė Marija, Daunoras Gintaras, Želvytė Rasa, Baliukonienė Violeta
Department of Food Safety and Quality, Faculty of Veterinary, Lithuanian University of Health Sciences, Tilzes Str. 18, LT-47181 Kaunas, Lithuania.
National Food and Veterinary Risk Assessment Institute, J. Kairiukscio Str. 10, LT-08409 Vilnius, Lithuania.
Toxins (Basel). 2024 Dec 30;17(1):13. doi: 10.3390/toxins17010013.
The degradation of zearalenone (ZEN) in the rumen of dairy cows is influenced by rumen pH, which is a key factor affecting this process. The aim of this study was to investigate the variation of ZEN in interaction with other mycotoxins at different ruminal pH environments (physiological (pH 6.5) and acidic (pH 5.5)) using an in vitro rumen model. Rumen fluid was collected from the caudoventral part of the rumen of cows using a pharyngeal-esophageal probe. To determine the changes in different mycotoxins (ZEN; AFLB; DON; T-2) in the rumen of cows, a model rumen system was used, and mycotoxins concentrations were detected by HPLC. The study found that at pH 6.5, ZEN alone and in combination with other mycotoxins (DON; T-2; AFLB) significantly ( < 0.05) reduced ZEN levels compared to the rumen environment at pH 5.5. It was observed that α-zearalenol (α-ZEL) and β-zearalenol (β-ZEL) concentrations were generally higher at a rumen pH of 6.5 compared to pH 5.5, averaging 47.09 µg/L and 35.23 µg/L, respectively. Additionally, the frequency of detection for both α-ZEL and β-ZEL was greater at pH 6.5 than at pH 5.5. A comparison of α-ZEL concentrations in rumen samples at pH 5.5 showed a 20% increase from the 6th to the 9th hour of the test, while β-ZEL levels remained unchanged over the same period.
玉米赤霉烯酮（ZEN）在奶牛瘤胃中的降解受瘤胃pH值影响，瘤胃pH值是影响这一过程的关键因素。本研究旨在使用体外瘤胃模型，研究在不同瘤胃pH环境（生理pH值（6.5）和酸性pH值（5.5））下，ZEN与其他霉菌毒素相互作用时的变化情况。使用咽-食管探头从奶牛瘤胃的尾腹部分采集瘤胃液。为了确定奶牛瘤胃中不同霉菌毒素（ZEN；黄曲霉毒素B1（AFLB）；脱氧雪腐镰刀菌烯醇（DON）；T-2毒素）的变化，采用了瘤胃模型系统，并通过高效液相色谱法检测霉菌毒素浓度。研究发现，在pH 6.5时，单独的ZEN以及与其他霉菌毒素（DON；T-2；AFLB）组合时，与pH 5.5的瘤胃环境相比，ZEN水平显著降低（<0.05）。观察到，与pH 5.5相比，瘤胃pH值为6.5时，α-玉米赤霉醇（α-ZEL）和β-玉米赤霉醇（β-ZEL）的浓度通常更高，平均分别为47.09 μg/L和35.23 μg/L。此外，pH 6.5时α-ZEL和β-ZEL的检测频率高于pH 5.5。对pH 5.5时瘤胃样品中α-ZEL浓度的比较显示，从试验第6小时到第9小时增加了20%，而同期β-ZEL水平保持不变。
