猪带绦虫和牛带绦虫卵的体外生化控制

In Vitro Biochemical Control of Taenia solium and Taenia saginata Eggs.

作者信息

de Souza Debora Castro, Puentes Lisseth Bibiana Figueroa, da Silva Adriane Toledo, Ferreira Dyesse Pollyane, Ferraz Carolina Magri, Braga Fabio Ribeiro, de Freitas Soares Filippe Elias

机构信息

Laboratório de Biotecnologia e Bioquímica Aplicada, Departamento de Química, Universidade Federal de Lavras, Lavras, MG, Brasil.

Laboratório de Parasitologia Experimental e Controle Biológico, Universidade Vila Velha, Vila Velha, ES, Brasil.

出版信息

Acta Parasitol. 2025 Jan 24;70(1):28. doi: 10.1007/s11686-024-00950-8.

DOI:10.1007/s11686-024-00950-8

PMID:39853400

Abstract

PURPOUSE

The present study evaluated in vitro the action of the plant protease papain (EC 3.4.22.2) on intact eggs of Taenia solium and Taenia saginata.

METHODS

Two experimental groups were composed for each of the gastrointestinal helminths: control group (G1) and treated group (G2). In each replicate corresponding to the control group, 100 µL of the suspension containing approximately 100 intact eggs (T. solium) or 50 eggs (T. saginata) and 100 µL of distilled water were added, while for the treated group 100 µL of the egg suspension and 100 µL of the papain solution at a concentration of 15% (m/v) were added. The experiment was conducted in four replicates. The groups were incubated in BOD at 28 ± 1 ºC in the dark for a period of 24 h.

RESULTS

The results demonstrated that there was a significant reduction (p < 0.01) of intact eggs, with average reduction percentages for T. saginata and T. solium of 74.36% and 64.29%, respectively.

CONCLUSIONS

This study is the first report on the in vitro control of helminth eggs (T. saginata and T. solium) using pure papain.

摘要

目的

本研究在体外评估了植物蛋白酶木瓜蛋白酶（EC 3.4.22.2）对猪带绦虫和牛带绦虫完整虫卵的作用。

方法

针对每种胃肠道蠕虫组建两个实验组：对照组（G1）和处理组（G2）。在与对照组对应的每个重复实验中，加入100 μL含有约100个完整虫卵（猪带绦虫）或50个虫卵（牛带绦虫）的悬浮液以及100 μL蒸馏水，而处理组则加入100 μL虫卵悬浮液和100 μL浓度为15%（m/v）的木瓜蛋白酶溶液。实验进行了四次重复。将各组在28 ± 1 ºC的黑暗环境中于生化培养箱中孵育24小时。