Bishoyi Ajit Kumar, Mandhata Chinmayee Priyadarsani, Sahoo Chita Ranjan, Samal Priyanka, Dubey Debasmita, Jali Bigyan Ranjan, Alamri Abdulaziz Mohammed, Khan Mohd Shahnawaz, Padhy Rabindra Nath
Central Research Laboratory, Institute of Medical Sciences and Sum Hospital, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, India.
Department of Clinical Hematology, Institute of Medical Sciences and Sum Hospital, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, India.
Cell Biochem Funct. 2025 Jan;43(1):e70043. doi: 10.1002/cbf.70043.
The biosynthesis of silver nanoparticles (AgNPs) using cyanobacteria has gained significant attention due to its cost-effective and eco-friendly advantages in green synthesis. Additionally, biogenic AgNPs show great potential for biological applications, particularly in combating infections caused by drug-resistant bacteria and fungi. This study synthesized using the cyanobacterium Oscillatoria salina (Os-AgNPs). The Os-AgNPs were characterized by a UV-vis spectral absorption peak at 447 nm, and their functional groups were identified through X-ray diffraction analysis, revealing a crystal structure with a 2θ value of 38°. Transmission electron microscopy (TEM) analysis showed an average nanoparticle size of 9.81 nm. The Os-AgNPs demonstrated remarkable antioxidant, antibacterial, and antifungal properties. Their antibacterial activity was tested against multidrug-resistant (MDR) Gram-positive bacteria, including Staphylococcus aureus, Streptococcus pyogenes, and Enterococcus faecalis, as well as Gram-negative bacteria such as Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, all isolated from clinical samples. The inhibition zones for bacterial strains ranged from 15 to 20 mm, as measured by the agar-well diffusion method. Similarly, the Os-AgNPs exhibited antifungal activity, with 20-30 mm inhibition zones against pathogenic fungi Trichophyton rubrum and Candida tropicalis. Additionally, the antiproliferative effects of the Os-AgNPs were evaluated on human cancer cell lines, including HeLa (cervical adenocarcinoma) and MD-AMB-231 (breast adenocarcinoma). In vivo toxicity studies were conducted using Swiss mouse models to assess the cytotoxic effects. Overall, the results suggest that Os-AgNPs, biosynthesized using O. salina, hold promise as potential antimicrobial and anticancer agents for pharmaceutical applications.
利用蓝细菌生物合成银纳米颗粒（AgNPs）因其在绿色合成中具有成本效益和环境友好的优势而备受关注。此外，生物合成的AgNPs在生物应用方面显示出巨大潜力，特别是在对抗由耐药细菌和真菌引起的感染方面。本研究使用盐生颤藻（Os-AgNPs）进行合成。Os-AgNPs在447nm处有紫外可见光谱吸收峰，通过X射线衍射分析确定了其官能团，揭示了2θ值为38°的晶体结构。透射电子显微镜（TEM）分析显示纳米颗粒的平均尺寸为9.81nm。Os-AgNPs表现出显著的抗氧化、抗菌和抗真菌特性。测试了它们对多重耐药（MDR）革兰氏阳性菌的抗菌活性，包括从临床样本中分离出的金黄色葡萄球菌、化脓性链球菌和粪肠球菌，以及革兰氏阴性菌如大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌。通过琼脂孔扩散法测量，细菌菌株的抑菌圈范围为15至20mm。同样，Os-AgNPs表现出抗真菌活性，对致病性真菌红色毛癣菌和热带假丝酵母的抑菌圈为20至30mm。此外，还评估了Os-AgNPs对人癌细胞系的抗增殖作用，包括HeLa（宫颈腺癌）和MD-AMB-231（乳腺腺癌）。使用瑞士小鼠模型进行体内毒性研究以评估细胞毒性作用。总体而言，结果表明利用盐生颤藻生物合成的Os-AgNPs有望作为潜在的抗菌和抗癌药物用于制药应用。
