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二维和三维培养模型中结肠癌细胞系的形态学和功能分析

Morphological and functional analysis of colorectal cancer cell lines in 2D and 3D culture models.

作者信息

Shinji Seiichi, Ogawa Yutaro, Yamada Takeshi, Matsuda Akihisa, Uehara Kay, Yokoyama Yasuyuki, Takahashi Goro, Iwai Takuma, Miyasaka Toshimitsu, Kanaka Shintaro, Hayashi Koki, Shichi Yuuki, Fujiwara Masakazu, Takahashi Kimimasa, Arai Tomio, Ishiwata Toshiyuki, Yoshida Hiroshi

机构信息

Department of Gastroenterological Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8603, Japan.

Division of Aging and Carcinogenesis, Research Team for Geriatric Pathology, Tokyo Metropolitan Institute for Geriatrics and Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 173-0015, Japan.

出版信息

Sci Rep. 2025 Jan 24;15(1):3047. doi: 10.1038/s41598-025-87660-x.

DOI:10.1038/s41598-025-87660-x
PMID:39856264
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11760527/
Abstract

The epithelial and mesenchymal features of colorectal adenocarcinoma (CRAC) cell lines were compared in two-dimensional (2D) and three-dimensional (3D) cultures. In 2D cultures, the three CRAC cell lines exhibited epithelial characteristics with high E-cadherin and low vimentin levels, whereas two exhibited mesenchymal traits with opposite expression patterns. In 3D cultures using low-attachment plates, mesenchymal cells from 2D cultures showed reduced vimentin mRNA levels. Morphologically, the five CRAC cell lines appeared similarly shaped in 2D culture but formed different structures in 3D culture. Epithelial DLD-1 and mesenchymal COLO-320 cells produced large granular spheres, whereas epithelial HCT-15 cells formed small solid spheres. Tubular structures were observed in epithelial CACO-2 and mesenchymal SW480 spheres. Desmosome-like structures developed in epithelial CRAC cells, whereas entosis was observed in CACO-2, HCT-15, and SW480 cells. The Ki-67-positive proliferating cell count varied in 2D and 3D cultures of epithelial cells but remained high and unchanged in mesenchymal cells. These findings suggest that while CRAC cells display distinct epithelial and mesenchymal properties in 2D cultures, they form diverse 3D structures, irrespective of these traits.

摘要

在二维（2D）和三维（3D）培养中比较了结直肠癌（CRAC）细胞系的上皮和间充质特征。在2D培养中，三种CRAC细胞系表现出上皮特征，E-钙黏蛋白水平高，波形蛋白水平低，而两种表现出具有相反表达模式的间充质特征。在使用低附着板的3D培养中，来自2D培养的间充质细胞的波形蛋白mRNA水平降低。形态学上，五种CRAC细胞系在2D培养中形状相似，但在3D培养中形成不同的结构。上皮性DLD-1细胞和间充质性COLO-320细胞产生大的颗粒球，而上皮性HCT-15细胞形成小的实心球。在上皮性CACO-2细胞球和间充质性SW480细胞球中观察到管状结构。在上皮性CRAC细胞中形成了桥粒样结构，而在CACO-2、HCT-15和SW480细胞中观察到了细胞内吞噬现象。Ki-67阳性增殖细胞计数在上皮细胞的2D和3D培养中有所不同，但在间充质细胞中保持高且不变。这些发现表明，虽然CRAC细胞在2D培养中表现出明显的上皮和间充质特性，但它们形成了不同的3D结构，与这些特性无关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/f768974abe9b/41598_2025_87660_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/bfa32dd1631d/41598_2025_87660_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/25afb4f3c58c/41598_2025_87660_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/337c3bdabc97/41598_2025_87660_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/6f756f998ffa/41598_2025_87660_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/83c4b3da361f/41598_2025_87660_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/02ddf779864d/41598_2025_87660_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/f768974abe9b/41598_2025_87660_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/bfa32dd1631d/41598_2025_87660_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/25afb4f3c58c/41598_2025_87660_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/337c3bdabc97/41598_2025_87660_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/6f756f998ffa/41598_2025_87660_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/83c4b3da361f/41598_2025_87660_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/02ddf779864d/41598_2025_87660_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0808/11760527/f768974abe9b/41598_2025_87660_Fig7_HTML.jpg

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