Lago-Sampedro Ana, Oualla-Bachiri Wasima, Maldonado-Araque Cristina, Valdés Sergio, González-Molero Inmaculada, Doulatram-Gamgaram Viyey, Delgado Elias, Chaves Felipe J, Castaño Luis, Calle-Pascual Alfonso, Franch-Nadal Josep, Rojo-Martínez Gemma, García-Serrano Sara, García-Escobar Eva
Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, Spain.
UGC Endocrinología y Nutrición, Hospital Regional Universitario de Málaga, IBIMA Plataforma BIONAND, 29590 Malaga, Spain.
Biomedicines. 2025 Jan 10;13(1):160. doi: 10.3390/biomedicines13010160.
: Although evidence suggests that miR-484 and several fruit components are involved in glucose metabolism and insulin resistance metabolic pathways, the relationship between serum miR-484 levels and fruit consumption in relation to the risk of Type 2 diabetes (T2DM) remains elusive. The aim of this study was to evaluate the possible association between serum miR-484 levels and fruit intake frequency with the risk of T2DM in the Spanish adult population. : 2234 subjects from the Di@bet.es cohort study without T2DM at baseline were studied. Socio-demographic, anthropometric and clinical data were recorded, as well as responses to a questionnaire on habits, including frequency of fruit consumption (daily vs. occasional). T2DM was diagnosed at baseline and after 7.5 years of follow-up. Baseline serum miR-484 levels were measured using real-time qPCR and categorized based on the 25th percentile. Association analyses were performed using logistic regression models adjusted for potential confounders. Interaction effects were evaluated on the multiplicative and additive scales. : There was no association between miR-484 levels and fruit intake frequency. Categorized miR-484 levels and fruit consumption were inversely and independently associated with the likelihood of incident T2DM. Analysis of the interaction effect suggests the presence of both positive multiplicative and additive interactions between miR-484 categories and fruit consumption frequency. : Our study demonstrates a protective effect of daily fruit intake and high miR-484 levels regarding the risk of T2DM and supports the nutritional recommendations advocating daily fruit consumption. This study also suggests that the combined effect of low miR-484 levels and occasional fruit intake may increase the risk of T2DM beyond their independent effects.
尽管有证据表明miR - 484和几种水果成分参与了葡萄糖代谢和胰岛素抵抗代谢途径，但血清miR - 484水平与水果摄入量之间与2型糖尿病（T2DM）风险的关系仍不明确。本研究的目的是评估西班牙成年人群中血清miR - 484水平和水果摄入频率与T2DM风险之间的可能关联。对来自Di@bet.es队列研究的2234名基线时无T2DM的受试者进行了研究。记录了社会人口统计学、人体测量学和临床数据，以及关于习惯的问卷调查的回答，包括水果消费频率（每日与偶尔）。在基线和7.5年的随访后诊断T2DM。使用实时定量PCR测量基线血清miR - 484水平，并根据第25百分位数进行分类。使用针对潜在混杂因素进行调整的逻辑回归模型进行关联分析。在乘法和加法尺度上评估交互作用。miR - 484水平与水果摄入频率之间没有关联。分类的miR - 484水平和水果消费与新发T2DM的可能性呈负相关且独立相关。交互作用分析表明，miR - 484类别与水果消费频率之间存在正向乘法和加法交互作用。我们的研究证明了每日水果摄入和高miR - 484水平对T2DM风险具有保护作用，并支持提倡每日食用水果的营养建议。这项研究还表明，低miR - 484水平和偶尔食用水果的联合作用可能会使T2DM风险增加，超过其独立作用。
