Wen Jiufu
Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
Hainan Engineering Research Center for Deep-Sea Aquaculture and Processing, Sanya 572018, China.
Genes (Basel). 2025 Jan 15;16(1):88. doi: 10.3390/genes16010088.
(Günther; 1867) is a member of the family Polynemidae. The placement of Polynemidae among teleosts has varied over the years.
Therefore, in this study, we sequenced the complete mitochondrial genome of , analyzed the characterization of the mitochondrial genome, and investigated the phylogenetic relationships of Polynemidae.
The length of the mitogenome was 16,738 bp, with a typical order. Nucleotide composition analysis showed that the mitogenome was AT-biased (54.15%), and the PCGs tended to use A and C rather than T and G at the third codon. All the PCGs started with the regular codon ATG, except for , which started with GTG. The termination codon varied across the PCGs. It was shown that the ka/ks ratios of all the PCGs were less than one. Phylogenetic analysis, based on the maximum likelihood (ML) and Bayesian inference (BI) methods, indicated that eight threadfins formed a well-supported monophyletic cluster. Polynemidae and Sphyraenidae clustered together as a monophyletic group. According to TimeTree analyses, the most recent common ancestor (MRCA) of Polynemidae was traced back to about 52.81 million years ago (MYA), while six species within Polynemidae diverged from 11.70 MYA to 20.05 MYA.
The present study provides valuable mitochondrial information for the classification of and new insights into the phylogenetic relationships of Polynemidae.
（冈瑟；1867年）是多鳍鱼科的成员。多年来，多鳍鱼科在硬骨鱼中的分类地位有所不同。
因此，在本研究中，我们对……的完整线粒体基因组进行了测序，分析了线粒体基因组的特征，并研究了多鳍鱼科的系统发育关系。
……线粒体基因组的长度为16,738 bp，具有典型的顺序。核苷酸组成分析表明，……线粒体基因组偏向于A和T（54.15%），并且蛋白质编码基因（PCGs）在第三个密码子处倾向于使用A和C而非T和G。除了……以GTG起始外，所有的PCGs均以常规密码子ATG起始。终止密码子在不同的PCGs中有所不同。结果表明，所有PCGs的ka/ks比值均小于1。基于最大似然法（ML）和贝叶斯推断法（BI）的系统发育分析表明，八种金线鱼形成了一个得到充分支持的单系类群。多鳍鱼科和魣科聚在一起形成一个单系类群。根据TimeTree分析，多鳍鱼科的最近共同祖先（MRCA）可追溯到约5281万年前（百万年前），而多鳍鱼科内的六个物种在1170万年前至2005万年前分化。
本研究为…… 的分类提供了有价值的线粒体信息，并为多鳍鱼科的系统发育关系提供了新的见解。
